CANCER CARIES, LIPID, CYSTIC FIBROSIS AND STRESS RESEARC

癌症龋齿、脂质、囊性纤维化和压力研究

• 批准号: 3514157
• 负责人: HARRIET R WILLIAMS
• 金额: $ 19.79万
• 依托单位: DELAWARE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3514157
• 关键词: 5 fluorouridine; all trans retinol; autogenic training; breast neoplasms; cystic fibrosis; drug adverse effect; enzyme substrate; human tissue; minority institution research support; neoplasm /cancer pharmacology; phospholipids; platinum; psychobiology; psychological stressor; pyrimidines; tocopherols; urinalysis; urine

The proposed work includes the following projects: 1) Effect of Pt-Pyrimidines on Mitotic and Enzyme Activity. Investigation of the effects of platinum-thymine and platinum-uracil on normal and cancer cells in Swiss white mice at different dose levels and time intervals; the effects of the drugs on chromosomes and spindle fibers of dividing normal and cancer cells; the inhibitory action of the drugs on alkaline and acid phosphatase activity in normal and cancer cells; and toxic effects of the drugs on normal and cancer cells of the intestine and kidney. 2) Pancreatic enzymes and their substrates. A chromatographic and histochemical study of the lipolytic activity of various vertebrate pancreas and duodenum under fed, fasted, and pilocarpine stimulated conditions. 3) Metabolites in urine of cystic fibrosis patients. Separation by high speed liquid-liquid chromatography and identification by gas chromatography/mass spectrometry, carbohydrate and amino acid analyses of unique metabolites in urine of cystic fibrosis patients and healthy humans. 4) Detection versus control of stress related variables. Determination of the relationship between detection and voluntary behavioral control of pulse wave velocity, peripheral blood flow (thermal), alpha brain wave frequencies, and skin potential changes.

拟开展的工作包括以下几个方面：1）铂-嘧啶类化合物的影响 有丝分裂和酶活性。 影响的研究 铂-胸腺嘧啶和铂-尿嘧啶对正常细胞和癌细胞的作用，瑞士白色 不同剂量水平和时间间隔的小鼠;药物对 分裂的正常细胞和癌细胞的染色体和纺锤体纤维; 药物对碱性和酸性磷酸酶活性的抑制作用 正常细胞和癌细胞;药物对正常和癌细胞的毒性作用 肠和肾的细胞。 2)胰酶及其底物。 用色谱和组织化学方法研究了不同脂肪酸的脂解活性 在进食、禁食和毛果芸香碱刺激下的脊椎动物胰腺和十二指肠 条件 3)囊性纤维化患者尿中的甲硫氨酸。 分离中 高速液-液色谱法及气相鉴别 色谱/质谱，碳水化合物和氨基酸分析的独特 囊性纤维化患者和健康人尿液中的代谢物。 四、 压力相关变量的检测与控制。 测定 脉搏波检测与自主行为控制的关系 速度，外周血流量（热），α脑波频率，和 皮肤电位变化。