THE ROLE OF THE NUCLEAR ENVELOPE IN INTRACELLULAR PROTEIN SORTING

在大多数真核生物有丝分裂过程中，分泌、受体 介导的内吞作用和受体循环停止。最多的 有丝分裂细胞的细胞内组织发生了显著的变化 核膜的丢失和有丝分裂的组装 纺锤形。核膜在细胞内转运中的作用 膜糖蛋白和一种细胞质蛋白正在研究中。 使用水疱性口炎病毒(VSV)的温度敏感突变体， 研究单糖蛋白的组装是可能的 病毒以同步方式传播。突变的蛋白质积累在 粗面内质网(RER)和核膜(NE)作为 不允许的温度。使用开发的单元格程序 去核，含有G蛋白的功能性核可以被移除 从受感染的细胞中分离出来，然后重新插入到另一个细胞中。保留 细胞在允许的温度下诱导分泌将允许 确定核包膜之间的通信程度， 粗面内质网等细胞器。 通过核所需的初级序列信息 进入细胞核的孔最近被定义为大T抗原 病毒SV-40的。由这个序列组成的合成肽正在被 用作探针，以识别涉及的核包膜成分 移位到核内部。

During mitosis in most eucaryotes, the processes of secretion, receptor mediated endocytosis, and receptor recycling come to a halt. The most striking alteration in the intracellular organization of mitotic cells is the loss of the nuclear envelope (NE) and the assembly of the mitotic spindle. The role of the nuclear envelope in intracellular trafficking of membrane glycoproteins and a cytoplasmic proteins is under investigation. Using a temperature sensitive mutant of Vesicular Stomatitis Virus (VSV), it is possible to study the assembly of the single glycoprotein of the virus in a synchronous fashion. The mutant protein accumulates in the Rough Endoplasmic Reticulum (RER) and Nuclear envelope (NE) as the non-permissive temperature. Using developed procedures for cell anucleation, functional nucllei containing the G protein can be removed from an infected cell and reinserted into a foreign cell. Retaining the cells to the permissive temperature to induce secretion will allow a determination of the extent of communication between the Nuclear envelope, Rough endoplasmic reticulum and other organeles. The primary sequence information required for passage through the nuclear pores into the nucleaus have recently been defined for the Large T-antigen of the virus SV-40. Synthetic peptides composed of this sequence are being used as probes, to identify nuclear envelope components involved in translocation to the nuclear interior.