MEMBRANE DAMAGE BY IMMUNE MECHANISMS

从大鼠大颗粒淋巴细胞瘤中提纯的胞浆颗粒 已有研究确定其在细胞毒和细胞毒性中的作用 这些淋巴细胞的其他功能。微生物的生化提纯 从颗粒中提取的细胞溶血素表明，它是一条单肽链。 大约65千克。当红细胞用溶血素裂解时，这个分子 在纯化后的膜中形成了一个大的孔状复合体。 细胞溶血素活性被多种脂类类似物阻断， 与膜插入机构一致。抗独特型抗体 针对磷酸胆碱结合的骨髓瘤蛋白TEPC 15提出了 显示与纯化的细胞溶血素结合并阻断其裂解活性， 说明细胞溶血素对胆碱有识别作用。生物活动 并对颗粒剂中的非溶细胞素成分进行了研究。确实是 显示DNA酶活性与Percoll中的LGL颗粒有关 渐变。这种活性不是细胞溶血素的一部分。核糖核酸酶活性 具有中性的最适pH值，在 非细胞毒性淋巴细胞。与其他实验室的合作研究引领了 用于其他颗粒成分的鉴定，包括一个因子，该因子是 向大颗粒淋巴细胞和其他细胞趋化，以及 嗜铬颗粒蛋白，一种先前仅在神经内分泌细胞中已知的抗原 颗粒。

The cytoplasmic granules purified from rat large granular lymphocyte tumors with NK activity have been studied to determine their role in cytotoxic and other functions of these lymphocytes. Biochemical purification of the cytolysin from the granules shows that it is a single peptide chain of about 65 kd. When erythrocytes are lysed with cytolysin, this molecule forms a large pore-like complex in the membrane which has been purified. Cytolysin activity was shown to be blocked by a variety of lipid analogues, in agreement with a membrane insertion mechanism. Anti-idiotype antibodies raised against the phosphorlycholine binding myeloma protein TEPC 15 were shown to bind to purified cytolysin and block its lytic activity, suggesting a choline recognition by cytolysin. The biological activities of non-cytolysin components of the granules were also studied. It was shown that a DNase activity is associated with LGL granules in the Percoll gradient. This activity was not part of the cytolysin. The DNAse activity has a neutral pH optimum and is found in much lesser amounts in non-cytotoxic lymphocytes. Collaborative studies with other labs have led to identification of other granule components, including a factor which is chemotactic to large granular lymphocytes and other cells, and chromogranin, an antigen previously known only in neuro-endocrine cell granules.