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CLONING AND CHARACTERIZATION OF HUMAN CYTOCHROME P-450

CLONING AND CHARACTERIZATION OF HUMAN CYTOCHROME P-450
人细胞色素 P-450 的克隆和表征
批准号：
4692334
负责人：
F J GONZALEZ
金额：
$0.0万
依托单位：
DIVISION OF CANCER EPIDEMIOLOGY AND GENETICS
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/4692334
关键词：
chemical carcinogenesis chemical structure function chromosome complement complementary DNA cytochrome P450 detoxification eukaryote gene expression genetic regulation liver messenger RNA methylcholanthrene molecular cloning molecular oncology nucleic acid sequence phenobarbital sex linked trait toxin metabolism

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英文摘要
Immunochemical methods are being employed to isolate human cytochrome P-450 genes. Cytochrome P-450s are purified from rat liver and extensively characterized with regard to inducivility by xenobiotics and steroids, and catalytic activity. Rabbit anti-cytochrome P-450 is then produced and utilized to screen human cDNA expression libraries. Full length cDNAs are isolated and their sequences are determined. These cDNAs are compared with their rodent P-450 counterparts. After sufficient characterization, P-450 cDNA probes will be used to analyze the regulation of cytochrome P-450 in cultured human cells and lymphocytes. In addition, these probes will be utilized to examine human genetic differences. As a model system we will first attempt to characterize the gene coding for the human debrisoquine 4-hydroxylase. The polymorphism of this enzyme system in humans has been well documented.
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FUNCTION OF XENOBIOTIC RECEPTORS
TRANSCRIPTIONAL REGULATION OF GENES ENCODING XENOBIOTIC METABOLIZING ENZYMES
FUNCTION OF XENOBIOTIC RECEPTORS
POLYMORPHIC DRUG OXIDATION--THE HUMAN AND RAT DEBRISOQUINE 4-HYDROXYLASE GENES
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