REGULATION OF SUGAR TRANSPORT AND METABOLISM IN LACTIC ACID BACTERIA

一种膜调控的糖-磷酸：磷酸水解酶已被纯化 并以干酪乳杆菌为特征菌株。这种酶的抗体有 已获得用于结构/功能实验。乳糖类似物 含有不能代谢的，(14C)标记的2-氟-D-葡萄糖或 用酶法制备了2-脱氧-D-葡萄糖。突变体 对乳酸链球菌进行了阳性耐药性和紫外线检测。 诱变程序。这些突变体缺乏依赖于ATP的葡萄糖激酶。 和/或PEP-甘露糖：磷酸转移酶活性，不能 依靠葡萄糖生长。通过使用细菌突变体，并运输 含(14C)-2F-乳糖和(14C)-2D-乳糖的研究，确凿证据 通过PTS的细胞内游离糖的磷酸化已经被 获得。消化链球菌的生化和生理学研究 厌氧菌已经透露，脯氨酸是运输所必需的 以及这种厌氧口腔病原体对葡萄糖的代谢。

A membrane-regulated, sugar-phosphate: phosphohydrolase has been purified and characterized from Lactobacillus casei. Antibodies to the enzyme have been obtained for use in structure/function experiments. Lactose analogs containing non-metabolizable, (14C)-labeled 2-fluoro-D-glucose or 2-deoxy-D-glucose have been prepared enzymatically. Mutants of Streptococcus lactis have been isolated by positive resistance and UV mutagenesis procedures. These mutants lack ATP-dependent glucokinase and/or PEP-mannose: phosphotransferase activities and are incapable of growth on glucose. Through the use of the bacterial mutants, and transport studies with (14C)-2F-lactose and (14C) 2D-lactose, definitive evidence for intracellular phosphorylation of free sugars via the PTS has been obtained. Biochemical, and physiological studies with Peptostreptococcus anaerobius have revealed that proline is a requirement for the transport and metabolism of glucose by this anaerobic oral pathogen.