ORIGIN OF FMRF-NH2 IMMUNOREACTIVITY IN RAT SPINAL CORD

Phe-Met-Arg-Phe-酰胺免疫反应性(FMRF-NH2-IR)高度集中 在大鼠脊髓后角，尤其是神经末梢 为了确定细胞胞体的位置。 感觉神经节和脊髓内含有FMRF-NH2-IR的I层终末 树根。FMRF-NH2-IR在两种组织中均有检测到，反相高效液相色谱法 分析表明，这两种组织含有相同的分子形式 也存在于脊髓中。腰-骶神经根切断术诱导50%的 脊髓腰段FMRF-NH2-IR降低， 这表明至少有一部分FMRF-NH2-IR存在于 组织是外周起源的。脊髓横断术 胸部正中平面导致腰椎FMRF-NH2-IR下降20%至50% 脊髓的节段，也表明存在 FMRF-NH2-IR呈下降通道。

Phe-Met-Arg-Phe-amide immunoreactivity (FMRF-NH2-IR) is highly concentrated in the dorsal horn of rat spinal cord, and particularly in nerve terminals of lamina I. In order to establish the location of the cell bodies of the lamina I terminals containing FMRF-NH2-IR in sensory ganglia and in spinal roots. FMRF-NH2-IR was found in both tissues, and reverse-phase HPLC analysis revealed that both tissues contain the same molecular forms that are also present in the spinal cord. Lumbo-sacral rhizotomy induced a 50% decrease of FMRF-NH2-IR in the lumbar segment of the spinal cord, suggesting that at least a portion of the FMRF-NH2-IR present in this tissue is of peripheral origin. Transection of the spinal cord at the midthoracic level induced a 20 to 50% decrease of FMRF-NH2-IR in the lumbar segment of the spinal cord, suggesting also that the presence of FMRF-NH2-IR in descending pathways.