ORIGIN OF FMRF-NH2 IMMUNOREACTIVITY IN RAT SPINAL CORD

大鼠脊髓 FMRF-NH2 免疫反应的起源

• 批准号: 4696425
• 负责人: C FERRARESE
• 金额: --
• 依托单位: NATIONAL INSTITUTE OF MENTAL HEALTH
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/4696425
• 关键词: amides; dorsal column; high performance liquid chromatography; human tissue; lumbosacral region; oligopeptides; reversed phase chromatography; spinal cord; spinal cord mapping; spinal cord surgery; spinal ganglion

Phe-Met-Arg-Phe-amide immunoreactivity (FMRF-NH2-IR) is highly concentrated in the dorsal horn of rat spinal cord, and particularly in nerve terminals of lamina I. In order to establish the location of the cell bodies of the lamina I terminals containing FMRF-NH2-IR in sensory ganglia and in spinal roots. FMRF-NH2-IR was found in both tissues, and reverse-phase HPLC analysis revealed that both tissues contain the same molecular forms that are also present in the spinal cord. Lumbo-sacral rhizotomy induced a 50% decrease of FMRF-NH2-IR in the lumbar segment of the spinal cord, suggesting that at least a portion of the FMRF-NH2-IR present in this tissue is of peripheral origin. Transection of the spinal cord at the midthoracic level induced a 20 to 50% decrease of FMRF-NH2-IR in the lumbar segment of the spinal cord, suggesting also that the presence of FMRF-NH2-IR in descending pathways.

Phe-Met-Arg-Phe-酰胺免疫反应性（FMRF-NH 2-IR）高度浓缩 在大鼠脊髓背角，特别是在神经末梢 第一层 为了确定细胞体的位置， 在感觉神经节和脊髓中含有FMRF-NH 2-IR的I层终末 根 FMRF-NH 2-IR在两种组织中均被发现， 分析显示，这两种组织含有相同的分子形式， 也存在于脊髓中。 腰骶神经根切断术诱导了50%的 脊髓腰段FMRF-NH 2-IR减少， 这表明存在于该组合物中的FMRF-NH 2-IR的至少一部分是在该组合物中存在的。 组织是外周起源的。 脊髓横断 胸中部水平诱导腰椎中FMRF-NH 2-IR降低20 - 50 脊髓节段，这也表明， FMRF-NH 2-IR在下行通路中。