Establishment of a physiological culture system to improve translation in metabolic research

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The major output from this will be technological. The ability to control the oxidative status of cells, combined with a more physiological cell culture media, will provide a powerful new tool for the study of adipose tissue biology. Furthermore, a deeper understanding of how cell lines respond to their environment has broad implications for other cellular models. This project will set standards for adipocyte models, improving reproducibility and translation between cells, mice and humans. Improved cellular models for studying metabolic diseases will benefit academic and industrial stakeholders. The fact our proposed technology is cheap and straightforward to implement makes it accessible to a global audience, not just those based in well-funded research environments. We will disseminate information through publication and also the Cambridge Metabolic Network Website to provide more detailed protocols. Research data management (RDM) will follow Findable, Accessible, Interoperable, and Re-usable (FAIR) principles.