NEW TESTING FOR ALLERGENIC EXTRACTS

A. 相对效价的ELISA：ELISA竞争试验已完成。 为过敏性提取物的标准化而开发。 最 过敏原提取物的制造商采用ELISA竞争 代替RAST抑制进行效价检测的试验。 与 通过实施该测定，我们降低了费用， 本实验室在过敏原检测中花费的人员时间 产品在其发布用于销售之前以及作为新的参考。 目前，研究正在进行测试桦树花粉，乳胶， 使用ELISA竞争法检测美洲、德国和东方蟑螂 比色法 B。 总蛋白的改良茚三酮测定法：改良茚三酮 开发并验证了用于检测过敏原中蛋白质的测定方法 萃取物 这种方法减少了人员的时间进行茚三酮 蛋白质超过一半。 过敏性产品的确认研究 检测实验室已显示茚三酮含量测定与 改良茚三酮测定法。 该方法被发送到制造部门， 其他测试实验室对两者相关性的投入 方法. 将对实验室的数据进行分析。 C. 化学发光法检测效价：ORIGEN免疫分析系统 被评价为等效或更好的效价测定方法 过敏性提取物的测试。 据确定，受限制的 一次可以测试的管的数量将导致较慢的 批次放行检测的周转时间。 D. 过敏性提取物的SDS-PAGE和蛋白质印迹法： 进行SDS-Page和Western印迹技术 可与等电聚焦结合使用或替代等电聚焦， 用于过敏原提取物和血清表征的BIEF-Light方法 过敏的人。

A. ELISA for Relative Potency: An ELISA Competition Assay has been developed for the standardization of allergenic extracts. Most manufacturers of allergenic extracts have adopted the ELISA Competition Assay in place of the RAST Inhibition for potency testing. With the implementation of this assay, we have reduced the expense and the personnel time incurred by this laboratory in the testing of allergenic products prior to their release for sale and as new references. Currently, studies are proceeding to test birch tree pollen, latex, and American, German, and Oriental cockroaches using the ELISA Competition Assay. B. Modified Ninhydrin Assay for Total Protein: A modified ninhydrin assay was developed and validated for testing protein in allergenic extracts. This method reduces personnel time for performing ninhydrin proteins by more than half. Validation studies by the Allergenic Product Testing Lab have shown good correlation between the Ninhydrin Assay and the Modified Ninhydrin Assay. The method was sent to manufacturing and other testing laboratories for their input into correlation of the two methods. Data from the laboratories will be analyzed. C. Chemiluminescence for Potency Testing: The ORIGEN Immunoassay System was evaluated as an equivalent or better method for performing potency testing of allergenic extracts. It was determined that the restricted number of tubes that could be tested at one time would cause a slower turn-around time for lot release testing. D. SDS-Page and Western Blotting of Allergenic Extracts: Studies are being conducted to determine if SDS-Page and Western blotting techniques can be used in conjunction with or to replace IsoElectric Focusing and BIEF-Light methods for characterization of allergenic extracts and serum from allergic individuals.