CHROMATIN STRUCTURE IN REGULATION OF MAMMALIAN GENE EXPRESSION
CHROMATIN STRUCTURE IN REGULATION OF MAMMALIAN GENE EXPRESSION
批准号:
5201910
负责人:
A DEAN
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
中文摘要
胚胎珠蛋白基因是-珠蛋白的第一个成员
英文摘要
The embryonic epsilon-globin gene is the first member of the beta-globin
family of genes to be expressed during development. Expression of the
beta-like-globin genes is under the control of the beta-globin locus
control region (LCR), encompassing erythroid specific DNase I
hypersensitive sites (HS) 5-18 kilobases upstream of the epsilon-globin
gene. The LCR has long range effects on the chromatin structure of the
beta-globin locus, and it activates transcription of the different globin
gene promoters as the genes are expressed sequentially during
development. Much of our current understanding of the process of gene
activation has been gained from in vitro studies where DNA is not
assembled with histones and other chromosomal proteins as it is in the
nucleus. To study the activation of a gene in its natural context of
chromatin in vivo we have used minichromosomes containing the human
epsilon-globin gene and portions of the beta globin LCR enhancer.
Expression of hue minichromosomal epsilon-globin gene in K562 human
erythroid cells is completely dependent on the presense if the beta-
globin LCR in cis. Using restriction endonuclease access, and indirect
end labelling experiments, we have observed that the promoter and 5'
proximal structural regions of the minichromosomal epsilon-globin gene
are covered by an array of positioned nucleosomes. Both types of studies
suggest alteration or loss of a single promoter-proximal nucleosome upon
activation of the gene for transcription. Regulated expression of the
epsilon-globin gene on the minichromsomes offers a means too study at
high resolution, the promoter-enhancer interaction of an activated gene
assembled in vivo into chromatin.
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CHROMATIN STRUCTURE IN REGULATION OF MAMMALIAN DEVELOPMENTAL GENE EXPRESSION
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批准号:3917369
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A DEAN
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依托单位:
CHROMATIN STRUCTURE IN REGULATION OF MAMMALIAN GENE EXPRESSION
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批准号:3839591
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A DEAN
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依托单位:
CHROMATIN STRUCTURE IN REGULATION OF MAMMALIAN GENE EXPRESSION
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批准号:3753954
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A DEAN
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依托单位:
CHROMATIN STRUCTURE IN REGULATION OF MAMMALIAN GENE EXPRESSION
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批准号:3875548
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A DEAN
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依托单位:
CHROMATIN STRUCTURE IN REGULATION OF MAMMALIAN GENE EXPRESSION
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批准号:3854542
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:A DEAN
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依托单位:
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