CHROMATIN STRUCTURE IN REGULATION OF MAMMALIAN GENE EXPRESSION
CHROMATIN STRUCTURE IN REGULATION OF MAMMALIAN GENE EXPRESSION
批准号:
5201910
负责人:
A DEAN
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
中文摘要
胚胎表型珠蛋白基因是β-珠蛋白的第一个成员。
在发育过程中要表达的基因家族。的表达
类β珠蛋白基因受β-珠蛋白基因控制
控制区(LCR),包括红系特异性DNA酶I
表珠蛋白上游5-18kb的超敏位点
吉恩。LCR对细胞的染色质结构有长程影响
β-珠蛋白基因座,它激活不同珠蛋白的转录
基因启动子作为基因在过程中顺序表达
发展。我们目前对基因过程的大部分理解
激活是从体外研究中获得的,而DNA不是
与组蛋白和其他染色体蛋白质组装在一起
原子核。研究基因在其自然环境中的激活
染色质在体内我们已经使用了含有人的微小染色体
Epsilon-珠蛋白基因和部分β珠蛋白LCR增强子。
HUE微小染色体表型珠蛋白基因在K562人中的表达
红系细胞完全依赖于存在,如果β-
顺式中的珠蛋白LCR。使用限制性内切酶访问,和间接
结束标记实验,我们观察到启动子和5‘
微染色体表型珠蛋白基因的近端结构区
被一系列定位的核小体所覆盖。这两种类型的研究
提示单个启动子-近端核小体的改变或丢失
激活基因以进行转录。受调控的基因表达
微小染色体上的Epsilon-珠蛋白基因也提供了一种研究手段
高分辨率,激活基因的启动子-增强子相互作用
在体内组装成染色质。
英文摘要
The embryonic epsilon-globin gene is the first member of the beta-globin
family of genes to be expressed during development. Expression of the
beta-like-globin genes is under the control of the beta-globin locus
control region (LCR), encompassing erythroid specific DNase I
hypersensitive sites (HS) 5-18 kilobases upstream of the epsilon-globin
gene. The LCR has long range effects on the chromatin structure of the
beta-globin locus, and it activates transcription of the different globin
gene promoters as the genes are expressed sequentially during
development. Much of our current understanding of the process of gene
activation has been gained from in vitro studies where DNA is not
assembled with histones and other chromosomal proteins as it is in the
nucleus. To study the activation of a gene in its natural context of
chromatin in vivo we have used minichromosomes containing the human
epsilon-globin gene and portions of the beta globin LCR enhancer.
Expression of hue minichromosomal epsilon-globin gene in K562 human
erythroid cells is completely dependent on the presense if the beta-
globin LCR in cis. Using restriction endonuclease access, and indirect
end labelling experiments, we have observed that the promoter and 5'
proximal structural regions of the minichromosomal epsilon-globin gene
are covered by an array of positioned nucleosomes. Both types of studies
suggest alteration or loss of a single promoter-proximal nucleosome upon
activation of the gene for transcription. Regulated expression of the
epsilon-globin gene on the minichromsomes offers a means too study at
high resolution, the promoter-enhancer interaction of an activated gene
assembled in vivo into chromatin.
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CHROMATIN STRUCTURE IN REGULATION OF MAMMALIAN DEVELOPMENTAL GENE EXPRESSION
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批准号:3917369
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负责人:A DEAN
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CHROMATIN STRUCTURE IN REGULATION OF MAMMALIAN GENE EXPRESSION
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批准号:3839591
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资助金额:$0.0万
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财政年份:--
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负责人:A DEAN
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CHROMATIN STRUCTURE IN REGULATION OF MAMMALIAN GENE EXPRESSION
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批准号:3753954
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负责人:A DEAN
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依托单位:
CHROMATIN STRUCTURE IN REGULATION OF MAMMALIAN GENE EXPRESSION
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批准号:3875548
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负责人:A DEAN
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依托单位:
CHROMATIN STRUCTURE IN REGULATION OF MAMMALIAN GENE EXPRESSION
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批准号:3854542
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资助金额:$0.0万
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财政年份:--
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负责人:A DEAN
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