NADPH REDUCTASES AND POLYOL PATHWAY IN OCULAR COMPLICATION

白细胞在糖尿病中的重要性是基于 细菌感染发生在高龄的证据 糖尿病患者的患病率及近期报告 暴露在高糖环境中的中性粒细胞 黏附于视网膜内皮细胞表面 毛细血管。因为它已经被确立为 糖醇过量积聚山梨醇引发 各种糖尿病并发症，如白内障形成 以及视网膜病变，即烟酰胺-腺嘌呤的还原形式 二核苷酸磷酸(NADPH)依赖的还原酶和 本文研究了狗白细胞中多元醇的形成。 多形核白细胞(PMN)和单核细胞 细胞(MNC)包含三种NADPH依赖的还原酶： 醛糖还原酶、醛还原酶和甘油醛 还原酶，以乙醛还原酶为主。尽管 极低水平的醛糖还原酶，葡萄糖 在中性粒细胞和中性粒细胞中通过山梨醇代谢成果糖 跨国公司。然而，糖醇的积累出现了。 在跨国公司中比在PMN中发生得更多。 饲喂半乳糖饲料的狗表现出蓄积 中性粒细胞和单核细胞中的半乳糖醇。这只半乳糖血症的狗 模型还发展了S等白细胞功能障碍。 NADPH氧化酶对中性粒细胞的诱导作用 据报道，淋巴细胞母细胞形成减少 人类糖尿病患者。

Importance of leukocytes in diabetes is based on the evidence that the bacterial infection occurs with h igh prevalence in patients with diabetes and the recent report that neutrophils exposed in a high glucose environment adhere to the surface of endothelial cells of retinal capillaries. Because it has been established th the accumulation of excess sugar alcohol sorbitol initiates various diabetic complications such as cataract formation and retinopathy, the reduced form of nicotinamide -adenine dinucleotide phosphate (NADPH)-dependent reductases and polyol formation in dog leukocytes was investigated. Both polymorphonuclear leukocytes (PMNs) and mononuclear cells (MNCs) contain three NADPH-dependent reductases: aldose reductase, aldehyde reductase, and glyceraldehyde reductase, with aldehyde reductase dominant. Despite the extremely low level of aldose reductase, glucose was metabolized into fructose through sorbitol in both PMNs and MNCs. However, the accumulation of sugar alcohols appeared to occur more in MNCs than PMNs. Dogs fed with galactose diet displayed accumulation of galactitol in both PMNs and MNCs. This galactosemic dog model also developed the leukocyte dysfunctions such s the impaired NADPH oxidase induction of neutrophils and the decreased lymphocyte blast formation that are reported with human diabetics.