CORE--ANIMAL AND CELL LINE MODELS

前列腺癌研究领域由于缺乏 良好的人类和动物模型。 迄今为止，来自人类的细胞系 原发性肿瘤尚未普遍获得，并且人转移性肿瘤 前列腺肿瘤细胞系LNCaP是唯一的 雄激素敏感 许多优秀的研究人员都尝试过，但没有 成功地从人类原发性前列腺肿瘤中获得细胞系。 只 三种人前列腺细胞系已被保藏在美国类型 培养物保藏中心（ATCC）储存库与26例人类乳腺癌相比 线 哥本哈根大鼠前列腺癌的Dunning大鼠模型 提供了关于前列腺癌生物学的有价值的信息， 科学界，但雄激素反应细胞系不能 在组织培养中生长。 现有的人类和大鼠前列腺癌 细胞系来源于完全转化的肿瘤， 一般来说，代表癌症远离恶性肿瘤的途径沿着 转型 很明显，各种各样的新前列腺癌 和前列腺组织细胞系，需要更广泛地覆盖 从正常细胞到永生化细胞的转化途径 细胞到非转移性肿瘤细胞到转移性肿瘤细胞， 雄激素依赖性和非依赖性。 这种缺乏普遍存在于 体外和体内模型也减缓了进展， 一种癌症不容易被转化为前列腺癌领域， 在前列腺癌模型中进行测试。 本动物和细胞系模型核心的目的是开发新的 前列腺组织和癌细胞系的质量和数量的提高 可供前列腺癌研究者使用的细胞系， 提供已知的前列腺肿瘤和细胞系模型， 研究人员进行测试。 具体而言，该模型核心将：（1） 建立和表征非永生化和永生化大鼠和人 体外前列腺上皮细胞、成纤维细胞和内皮细胞系 来源于正常和癌组织来源。 我们将分发 这些新的细胞系的其他孢子和非孢子研究人员， 以及ATCC的一般分布， 细胞系资源，以科学界作为一个整体，和（2） 建立大鼠和人前列腺癌模型， SPORE研究人员测试新的预防和治疗方法 利用新的和已建立的细胞系的策略。

The field of prostate cancer research has suffered because of the lack of good human and animal models. To date, a cell line derived from a human primary tumor has not been generally available and the human metastatic prostate tumor cell line LNCaP is the only tumor cell line that is androgen responsive. Multiple good investigators have attempted, without success, to derive cell lines from human primary prostate tumors. Only three human prostate cell lines have been deposited in the American Type Culture Collection (ATCC) repository as compared to 26 human breast cancer lines. The Dunning rat model of prostate cancer in the Copenhagen rat has provided valuable information about the biology of prostate cancer to the scientific community, but an androgen-responsive cell line can not be grown in tissue culture. Both the available human and rat prostate cancer cell lines are derived from tumors which are fully transformed and, in general, represent cancers far along the pathway of malignant transformation. It is clear that a wide variety of new prostate cancer and prostate tissue cell lines are needed which more extensively cover the gamut of the transformation pathway from normal cells to immortalized cells to nonmetastatic tumor cells to metastatic tumor cells, both androgen dependent and independent. This lack of generally available in vitro and in vivo models also slows progress since ideas and advances in one cancer are not readily translated to the field of prostate cancer by testing in prostate cancer models. The purpose of this Animal and Cell Lines Models Core is to develop new prostate tissue and cancer cell lines to enhance the quality and quantity of available cell lines for use by prostate cancer investigators and to make available the known prostate tumor and cell line models to researchers for testing. Specifically, this Models Core will: (1) Establish and characterize non immortalized and immortalized rat and human prostate epithelial, fibroblast, and endothelial cell lines in vitro derived from both normal and cancerous tissue sources. We will distribute these new cell lines to other SPORE and non-SPORE investigators as requested as well as to the ATCC for general distribution as established cell line resources to the scientific community as a whole, and (2) Establish rat and human prostate cancer models for both SPORE and non- SPORE investigators for the testing of new prevention and treatment strategies utilizing both new and established cell lines.