ULTRABITHORAX REGULATION OF CONNECTIN

本项目的目标是确定果蝇胚胎中同源异型基因Ultrabithorax（Ubx）如何调控细胞粘附蛋白连接蛋白（Con）。 拟进行的实验将解决两个具体目标：（1）表征Ubx和Con蛋白在胚胎神经系统中的正常分布模式，以及（2）通过操纵Ubx表达来检查Con的调节。 我将使用功能丧失或异位表达不同蛋白质同种型的胚胎，这些蛋白质同种型是Ubx基因的产物。 免疫组织化学和共聚焦显微镜将用于检查Con表达和神经系统形态学的变化。 拟议的项目将增加知识体系的同源异型基因和细胞粘附分子在神经系统的发展中的作用。

The goal of this project is to determine how Connectin (Con), a cell adhesion protein, is regulated by the homeotic gene Ultrabithorax (Ubx) in the Drosophila embryo. The proposed experiments will address two specific aims: (1) to characterize the normal patterns of Ubx and Con protein distribution in the embryonic nervous system, and (2) to examine the regulation of Con by manipulating Ubx expression. I will use embryos that have loss-of-function or ectopic expression of the different protein isoforms that are products of the Ubx gene. Immunohistochemistry and confocal microscopy will be used to examine changes in Con expression and in nervous system morphology. The proposed project will augment the body of knowledge of the roles of homeotic genes and cell adhesion molecules in the development of the nervous system.