ULTRABITHORAX REGULATION OF CONNECTIN

CONNECTIN 的 ULTRABITHORAX 调节

• 批准号: 6056671
• 负责人: Stephanie R Songer
• 金额: $ 3.17万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-28 至 2000-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6056671
• 关键词: Drosophilidae; arthropod genetics; cell adhesion molecules; confocal scanning microscopy; developmental genetics; developmental neurobiology; embryology; gene induction /repression; homeobox genes; immunocytochemistry; invertebrate embryology; neurogenesis; neurogenetics

The goal of this project is to determine how Connectin (Con), a cell adhesion protein, is regulated by the homeotic gene Ultrabithorax (Ubx) in the Drosophila embryo. The proposed experiments will address two specific aims: (1) to characterize the normal patterns of Ubx and Con protein distribution in the embryonic nervous system, and (2) to examine the regulation of Con by manipulating Ubx expression. I will use embryos that have loss-of-function or ectopic expression of the different protein isoforms that are products of the Ubx gene. Immunohistochemistry and confocal microscopy will be used to examine changes in Con expression and in nervous system morphology. The proposed project will augment the body of knowledge of the roles of homeotic genes and cell adhesion molecules in the development of the nervous system.

该项目的目标是确定在果蝇胚胎中，细胞黏附蛋白连接素(CON)是如何受同源异型基因UBX(UBX)调控的。这些实验将针对两个特定的目标：(1)研究胚胎神经系统中Ubx和Con蛋白的正常分布模式；(2)通过调控Ubx的表达来研究Con的调节。我将使用功能丧失或异位表达Ubx基因产物的不同蛋白质亚型的胚胎。免疫组织化学和共聚焦显微镜将用于检测CON表达和神经系统形态的变化。这项拟议的项目将增强同源异型基因和细胞黏附分子在神经系统发育中的作用的知识体系。