GENE EXPRESSION IN AGING BY HIGH DENSITY ARRAY ANALYSIS

DNA阵列杂交技术将用于创建衰老细胞和组织中定量基因表达变化的数据库。在第一阶段的研究中，将开发和验证使用尼龙DNA阵列进行高通量基因表达分析的方法，建立激光捕获显微解剖组织构建探针的可行性，并证明使用这些数据检测年龄相关基因表达差异的可行性。LifeSpan biosciences随后将把该数据库作为基因表达和衰老的信息资源，向学术界和药学界进行市场营销。在衰老过程中差异表达的基因的发现将最终提供信息，用于设计与年龄相关的变性和疾病的治疗干预药物。拟议的商业应用：由此产生的基因表达数据库将作为商业数据库产品直接销售给学术界和工业科学界。此外，疾病相关衰老基因的识别将为诊断和治疗衰老相关疾病的发展提供机会。

DNA array hybridization technology will be used to create a database of quantitative gene expression changes in aging cells and tissues. During the Phase I study, methods for high throughput gene expression analysis using Nylon based DNA arrays will be developed and validated, the feasibility of constructing probes from laser capture microdissected tissues will be established, and the feasibility of using this data to detect differences in age-related gene expression will be demonstrated. LifeSpan will then market access to this database to the academic and pharmaceutical communities as a resource for information on gene expression and aging. The discovery of genes that are differentially expressed in aging will ultimately provide information that will be used in the design of agents for therapeutic intervention in age-related degeneration and disease. PROPOSED COMMERCIAL APPLICATIONS: The resulting database of gene expression will be marketed directly to the academic and industrial scientific community as a commercial database product. In addition, the identification of disease-associated aging genes will result in opportunities for the development of diagnostics and therapeutics to treat aging related diseases.