CORE--MOLECULAR BIOLOGY

分子生物学核心将用于子项目1、2、4和6。 的 核心将提供服务，将大大促进研究 第1和第6项建议，更重要的是， 次级项目2和4获得自己没有的方法 laboratories. 获得这一核心至关重要，因为 这些技术在每个研究者的实验室里都是禁止的， 贵了 例如，Coronado博士（子项目2）希望获得 可靠和丰富的供应某些毒素，从墨西哥面包， 这是一种蜥蜴，它将被用作解剖 Ryanodine受体在心肌中的功能。 服务 分子生物学核心提供的技术将包括分离毒素 cDNA，确定DNA序列和构建表达载体 这将允许细菌产生毒素。 表达 这些重组毒素的纯化将通过蛋白质 生化核心（核心B）。 科罗纳多医生既没有 设备或专业知识，使其能够在自己的实验室中完成。 然而，使用这两项核心服务将使该项目能够 及时完成。

The Molecular Biology Core will be used by subprojects 1, 2, 4, and 6. The core will provide services that will greatly facilitate the research proposed by subprojects 1 and 6, and more importantly, the core will allow subprojects 2 and 4 access to methodologies absent from there own laboratories. Access to such a core is essential because implementation of these technologies in each investigators' laboratory would be prohibitively expensive. For example, Dr. Coronado (subproject 2) wishes to obtain a reliable and abundant supply of certain toxins, from the Mexican breaded lizard Heloderma horridum horridum, that will be used as tools to dissect the function of the ryanodine receptor in cardiac muscle. Services provided by the Molecular Biology Core will include isolating the toxin cDNAs, determining the DNA sequence and constructing expression vectors that will permit the production of the toxins in bacteria. The expression and purification of these recombinant toxins will be done by the Protein Biochemistry Core (Core B). Dr. Coronado does not have either the equipment or the expertise to allow this to be done in his own laboratory. However, the use of the two core services will enable this project to be completed in a timely manner.