CONSTRUCTION OF A NATIONAL RESOURCE FOR ZEBRAFISH

在过去的几年里，我们对 脊椎动物的发育，很大程度上是基于几个遗传模型的工作 有机体。斑马鱼是这些模式生物中最新的一种。因为 人体图案的基本原理在 所有脊椎动物的胚胎发生，从胚胎工作中获得的见解 斑马鱼将对人类健康和疾病产生影响。此外， 对这种生物的研究符合《动物福利法》的意图 因为许多高等脊椎动物的用途现在可以用这种 低等脊椎动物。 俄勒冈大学开始对斑马鱼进行系统的遗传学研究 多年来，尤金是唯一一个表演这种音乐的地方。最近， 然而，国际上对这种有机体的兴趣已经大大增加； 斑马鱼的胚胎学和遗传学研究及其最新进展 对超过7000个基因突变的识别导致了戏剧性的 越来越多的实验室使用这种微生物来研究 脊椎动物发育的基本机制。目前，这些遗传库 分布在28个国家的100多个实验室。使 为新的突变体留出空间，实验室必须停止他们目前的一些 股票，其中许多是永久丢失的。斑马鱼的研究 社区迫切需要一个集中的站点来保存和跟踪 并便于将其分发给研究人员，因此 支持和促进研究机会的整体预防 重复劳动。 我们建议建造一个设施来维持野生和突变种群 并将这些种群广泛提供给 国际研究界。需要一个库存中心，因为它可以 取消个别实验室维持库存的要求 他们无法学习，它可以提供比提供动物更低的成本 单个实验室，最重要的是，它可以确保更高的 质量和一致性的可能水平。具体来说，我们建议： 1.建立库存中心，作为中央储备库 材料和信息。 2.向研究界广泛提供信息。 3.制定增进健康的方法。

The last several years have witnessed an explosion in our understanding of vertebrate development, largely based on work from a few model genetic organisms. The zebrafish is the newest of these model organisms. Because the basic principles of body patterning appear similarly during embryogenesis of all vertebrates, insights gained from work on embryonic zebrafish will have implications for human health and disease. Moreover, research on this organism meets the intent of the Animal Welfare Act because use of many higher vertebrates can now be replaced by use of this lower vertebrate. Systematic genetic research on zebrafish began at the University of Oregon and for many years Eugene was the only place it was performed. Recently, however, international interest in this organism has grown tremendously; studies of the embryology and genetics of zebrafish and the recent identification of over 7,000 genetic mutations have led to a dramatic increased in the number of laboratories using this organism to study the basic mechanisms of vertebrate development. Currently these genetic stocks are distributed among more than 100 laboratories in 28 countries. To make room for new mutants, laboratories must discontinue some of their current stocks many of which are permanently lost. The zebrafish research community desperately needs a centralized site to preserve and keep track of these stocks and facilitate their distribution to researchers, thus supporting and promoting research opportunities whole preventing duplication of effort. We propose to construct a facility to maintain wild-type and mutant stocks of zebrafish and to make these stocks widely available to the international research community. A stock center is needed because it can eliminate the requirement of individual laboratories to maintain stocks they are unable to study, it can provide animals at lower cost than individual laboratories, and most importantly, it can ensure the higher possible levels of quality and uniformity. Specifically we propose: 1. To establish a stock center to serve as a central repository for materials and information. 2. To make information widely available to the research community. 3. To develop methods for improving health.