QUANTITATION OF CARDIAC MYOCYTE CONTRACTILE DYNAMICS

描述：（改编自申请人的摘要）：一些基本的 问题旨在了解的生理学和病理生理学的 心肌现在在细胞水平上得到解决。的主要目的 第二阶段的应用是将收缩性测量工具商业化 在I期期间开发用于心肌细胞的研究。的 用于定量肌细胞长度和肌节的数字成像方法 间隔在迄今为止所做的实验室测试中表现得非常好。的 研究者提出了一些从心脏病患者中收集数据的例子， 心肌细胞以及来自非心脏组织的细胞， 变化这些实验结果证明了光学的可行性 尺寸确定方法及其在设计中的实现能力。的 研究人员已经获得了六个已建立的心肌研究实验室， 作为我们系统的后原型版本的评估站点。四 其他实验室将申请Ionoptix Corp.的尺寸标注工具， 包括微血管、肾小管和成骨细胞的组织阵列。 这里所代表的研究兴趣的范围表明， 市场的细胞尺寸系统。最后，这些增强 第二阶段的系统，包括全面的合同， 瞬态分析和同时荧光离子定量将进一步 这种仪器的商业吸引力。 拟议商业应用：不可用

DESCRIPTION : (Adapted from applicant's abstract): Some of the fundamental questions aimed at understanding the physiology and pathophysiology of the myocardium are now being addressed at the cellular level. The principal aim of this Phase II application is to commercialize contractility measurement tools developed during the Phase I period for the study of cardiac myocytes. The digital imaging approach for quantitation of myocyte cell length and sarcomere spacing has performed extremely well in laboratory tests done thus far. The investigators present a number of examples of data collected from cardiac myocytes as well as from non-cardiac tissues which undergo rapid dimensional changes. These experimental results demonstrate the feasibility of the optical dimensioning approach and the power of its implementation in the design. The investigators have secured six established cardiac muscle research labs to serve as evaluation sites for post-prototype versions of our systems. Four additional labs will apply Ionoptix Corp.'s dimensioning tools to a diverse array of tissues including blood micro vessels, renal tubules and osteolasts. The range of research interest represented here indicates a broad commercial market for the cell dimensioning systems. Finally, the enhancements to these systems that is proposed for Phase II, including comprehensive contractile transient analysis and simultaneous fluorescence ion quantitation will further the commercial appeal of this instrumentation. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE