CYCLIN DEPENDENT KINASE 5 IN CANINE MOTOR NEURON DISEASE

描述（改编自申请人摘要）：Sherril L.绿色，DVM， 博士，是ACVIM的文凭，曾担任临床实验室 在过去的两年里，他一直是斯坦福大学的动物兽医。 她 在神经科学博士培训与理查德Vulliet博士在UCD专注于 tau蛋白磷酸化和蛋白激酶CDK5在啮齿类动物模型中的作用 中风 自从完成博士学位后，绿色博士一直从事 临床实验动物护理，特别是大型动物的重症监护 物种 绿色博士计划利用拟议的研究，以扩大她的 在神经科学和蛋白质生物化学的背景，并发展技能， 分子生物学，同时追求她在HCSMA的神经生物学的兴趣， 运动神经元疾病的动物模型。 博士绿色的研究是共同赞助的琳达科克博士，DVM，博士，一个 比较神经病理学家谁第一次建立HCSMA殖民地，和博士。 James Ferrell，医学博士，博士，生物化学家，以其MAP工作而闻名 激酶和细胞周期。 绿色博士将在两家公司的实验室工作 博士科克拥有一个设备齐全的实验室，专门研究 比较神经病理学，和法雷尔博士，谁有一个装备精良的 生物化学实验室 绿色博士将评估CDK 5参与 HCSMA。 她的初步数据显示， 动物脊髓样品中CDK5的活性和蛋白水平 是纯合子的 她提出的实验将解决三个问题 基本问题：1）为什么HCSMA中CDK5比活性增加？ 2)为什么HCSMA中CDK5蛋白水平升高？（3）CDK5 磷酸化位点的神经丝占据HCSMA？

DESCRIPTION (Adapted from the applicant's abstract): Sherril L. Green, DVM, PhD, is a Diplomate of the ACVIM and has served as a clinical laboratory animal veterinarian at Stanford University for the past two years. Her doctoral training in neuroscience with Dr. Richard Vulliet at UCD focused on tau protein phosphorylation and the protein kinase CDK5 in rodent models for stroke. Since completing her PhD degree, Dr. Green has been engaged in clinical laboratory animal care, especially intensive care for large animal species. Dr. Green plans to use the proposed studies to expand her background in neuroscience and protein biochemistry and to develop skills in molecular biology while pursuing her interests in the neurobiology of HCSMA, an animal model for motor neuron disease. Dr. Green's research is co-sponsored by Dr. Linda Cork, DVM, PhD, a comparative neuropathologist who first established the HCSMA colony, and Dr. James Ferrell, MD, PhD, a biochemist who is well known for his work on MAP kinase and the cell cycle. Dr. Green will work in the laboratories of both Dr. Cork, who has a fully equipped laboratory that specializes in comparative neuropathology, and Dr. Ferrell, who has a well-equipped biochemistry laboratory. Dr. Green will evaluate the involvement of CDK5 in HCSMA. Her preliminary data demonstrate alterations in both the specific activity and protein levels of CDK5 in spinal cord samples from animals homozygous for the disease. Her proposed experiments will address three fundamental questions: 1) Why is CDK5 specific activity increased in HCSMA? 2) Why are CDK5 protein levels increased in HCSMA?, and 3) Are CDK5 phosphorylation sites on neurofilaments occupied in HCSMA?