BEHAVIORAL REGULATION OF NEUROENDOCRINE CELLS

神经内分泌细胞的行为调节

• 批准号: 2889862
• 负责人: Emilie F. Rissman
• 金额: $ 7.78万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-15 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2889862
• 关键词: Mammalia; behavioral /social science research tag; biological models; cues; denervation; gene expression; gonadotropin releasing factor; immunocytochemistry; in situ hybridization; neuroendocrine system; olfactory stimulus; ovariectomy; ovulation; reproduction; sensory mechanism; sex behavior; stereotaxic techniques

DESCRIPTION (Adapted from applicant's abstract): This is a request for a RSDA level II award. The research investigates the affects of behavioral interactions on neuroendocrine cell functioning. Specifically the candidate will examine the impact of interactions with males on neurons that produce gonadotropin-releasing hormone (GnRH) in the female musk shrew (Suncus murinus). Musk shrews, unlike rodents, have no estrous cycle and most aspects of reproduction are induced by contact with males. Mating causes a dramatic (50%) increase in the numbers of cells that contain gonadotropin-releasing hormone immuno-reactivity (GnRH-ir) in the brain. This change occurs in GnRH-ir cells located in the olfactory bulb and rostral forebrain within 15 minutes after the females are introduced to males. Fifteen hours later significant increases in GnRH peptide content can be measured in the preoptic area, median eminence, and several other brain nuclei. This is accompanied by increased numbers of GnRH-ir cells in most brain regions. Cell numbers and GnRH content throughout the brain continue to increase in animals that do not ovulate, but drop in females that ovulate in response to a single ejaculation. The data suggests that behavior triggers increased production of GnRH peptide in specific cell bodies. This highly plastic and dramatic response of GnRH-containing neurons to specific behavioral cues has never been reported before in a mammal. The aims of these studies are to characterize the sensory/behavioral inputs that are required to activate GnRH neurons. To accomplish this goal the candidate will conduct a series of behavioral studies and then develop the required new surgical methods to denervate selected sensory input pathways. Once the candidate has specified the cues that affect neuroendocrine functioning the candidate will employ them to determine whether behavior affects the transcription of new genetic messages. She will employ in situ hybridization and immunocytochemistry to accomplish these aims. It is well known that behavioral interactions and emotional state can affect reproductive status. How behaviors actually affect the neurons that control neuroendocrine function is not well understood. The proposed experiments would establish a model to assess the direct impact of behavior on the molecular functioning and expression of cells that regulate reproduction in all mammals via their ability to produce and secrete GnRH. RSDA support will free the candidate from teaching and administrative duties. The release time will be used to acquire new skills in the laboratories of two colleagues that are experts in these areas. The goals of this proposal can only be attained if the candidate received the needed training in modern neurobiological and molecular techniques.

描述（改编自申请人的摘要）：这是一个请求， RSDA二级奖。 研究调查了行为的影响， 神经内分泌细胞功能的相互作用。 特别是候选人 将研究与雄性的相互作用对神经元的影响， 雌麝鼩的促性腺激素释放激素（GnRH murinus）。 与啮齿动物不同，麝香鼩没有发情周期， 生殖的各个方面都是通过与雄性的接触来诱导的。 交配导致 细胞数量急剧增加（50%）， 促性腺激素释放激素免疫反应性（GnRH-ir）在大脑中。 这种变化发生在位于嗅球的GnRH-ir细胞中， 在雌性动物被引入后15分钟内， 男性。 15小时后GnRH肽含量显著增加 可以在视前区、正中隆起和其他几个区域测量 脑核团 这伴随着GnRH-ir细胞数量的增加， 大部分脑区 整个大脑的细胞数量和GnRH含量 在不排卵的动物中继续增加，但在雌性动物中下降 对一次射精作出反应而排卵 数据表明 行为触发特定细胞中GnRH肽的产生增加 尸体 这种高度可塑性和戏剧性的反应， 神经元对特定行为线索的反应在以前从未报道过， 哺乳动物。 这些研究的目的是描述 感觉/行为输入，需要激活GnRH神经元。 到 为了实现这一目标，候选人将进行一系列的行为 研究并开发所需的新手术方法来切除神经 选择感官输入路径。 一旦候选人指定了线索 影响神经内分泌功能，候选人将利用它们来 确定行为是否影响新基因的转录， 充分和传播 她将采用原位杂交和免疫细胞化学技术， 实现这些目标。 众所周知，行为互动和 情绪状态会影响生殖状况 行为实际上 影响控制神经内分泌功能的神经元 明白 拟议的实验将建立一个模型，以评估 行为对分子功能和表达的直接影响 所有哺乳动物的生殖细胞都是通过产生 并分泌促性腺激素释放激素 RSDA的支持将使候选人从教学中解放出来， 行政职责。 释放时间将用于获得新技能 在这些领域专家的两位同事的实验室中进行。 的 只有当候选人收到了 需要接受现代神经生物学和分子技术的培训。