ROLE FOR FKBP12 IN GNRH SIGNALING

描述：(根据申请者的描述改编)本研究基于 假设在促性腺激素细胞中，FKBP12可能是一种 IP3受体的调节蛋白，并通过GnRH信号调控。 该提案的总体目标是评估FKBP12在 IP3受体功能的调节和促性腺激素释放激素诱导的促黄体生成素分泌这个 研究建议量化FKBP12在促性腺激素释放激素诱导的黄体生成素中的作用 使用体外和体内模型进行分泌并确定 IP3-受体复合体的表达、组装或磷酸化 由荷尔蒙调节，改变对GnRH的敏感性。 项目应提供有关GnRH刺激的PKC的信息 导致内质网钙释放的途径。会的 使用药物FK506来测试FKBP12的作用。FK506是一款 广泛用于器官移植的免疫抑制药物。因此， 关于其在生殖系统中的潜在作用的信息也将 将被获得。

DESCRIPTION: (Adapted from applicant's description) This research is based on the hypothesis that, in gonadotropes, a cellular protein, FKBP12 may be a regulatory protein for IP3 receptors and be controlled via GnRH signaling. The broad goal of the proposal is to evaluate the role of FKBP12 in the regulation of IP3 Receptor function and GnRH-induced LH secretion. The study proposes to quantify the contribution of FKBP12 to GnRH-induced LH secretion using in vitro and in vivo models and to determine whether expression, assembly, or phosphorylation of the IP3-Receptor complex is regulated by hormones that alter sensitivity to GnRH. The projects should provide information about the GnRH stimulated PKC pathway leading to calcium release from the endoplasmic reticulum. It will use a drug, FK506, to test the role of FKBP12. FK506 is an immunosuppressive drug that is widely used in organ transplants. Thus, information about its potential role in the reproductive system would also be obtained.