HIGH THROUGHPUT MICROFLUIDIC CHIP BASED DNA SYNTHESIZER

基于高通量微流控芯片的 DNA 合成器

• 批准号: 6144565
• 负责人: SURENDRA CHATURVEDI
• 金额: $ 9.93万
• 依托单位: ORCHID BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-05-10 至 2001-11-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6144565
• 关键词: DNA; DNA primers; RNA; biomedical automation; biomedical equipment development; fluid flow; high throughput technology; microprocessor /microchip; nucleic acid chemical synthesis; nucleic acid hybridization; nucleic acid sequence

The sequencing of the human genome has stimulated the demands for DNA synthesis for hybridization, amplification and sequence analysis. Common DNA probes of primers are used once or twice, but typical methods for their synthesis prepares many thousand reactions. Orchid Biocomputer Inc. has prepared microfluidic chips and instrumentation for chemical synthesis. These devices allow minimal consumption of reagents for the preparation of 96 or 384 compounds from solid supports. It is the object of this grant to develop and adapt this microfluidic technology for the high throughput preparation of DNA in a cost-effective manner.

人类基因组的测序刺激了对用于杂交、扩增和序列分析的DNA合成的需求。引物的普通DNA探针使用一次或两次，但其合成的典型方法准备了数千个反应。 Orchid Biocomputer Inc.已经为化学合成准备了微流控芯片和仪器。 这些装置允许从固体支持物制备96或384种化合物的试剂消耗最少。 这项资助的目的是开发和调整这种微流体技术，以具有成本效益的方式高通量制备DNA。