HIGH THROUGHPUT MICROFLUIDIC CHIP BASED DNA SYNTHESIZER

人类基因组的测序刺激了对DNA合成的需求，用于杂交、扩增和序列分析。普通的DNA探针会使用一次或两次，但它们的典型合成方法需要准备数千个反应。兰花生物计算机公司已经准备了用于化学合成的微流控芯片和仪器。这些设备允许从固体载体制备96或384种化合物所需的试剂消耗最少。这笔赠款的目的是开发和调整这种微流控技术，以经济高效的方式高通量地制备DNA。

The sequencing of the human genome has stimulated the demands for DNA synthesis for hybridization, amplification and sequence analysis. Common DNA probes of primers are used once or twice, but typical methods for their synthesis prepares many thousand reactions. Orchid Biocomputer Inc. has prepared microfluidic chips and instrumentation for chemical synthesis. These devices allow minimal consumption of reagents for the preparation of 96 or 384 compounds from solid supports. It is the object of this grant to develop and adapt this microfluidic technology for the high throughput preparation of DNA in a cost-effective manner.