HIGH THROUGHPUT MICROFLUIDIC CHIP BASED DNA SYNTHESIZER

人类基因组的测序刺激了对DNA合成的需求，用于杂交、扩增和序列分析。普通的引物DNA探针只使用一到两次，但它们的典型合成方法需要进行数千次反应。兰花生物计算机公司为化学合成准备了微流控芯片和仪器。这些装置允许从固体载体制备96或384种化合物的试剂消耗最少。这项拨款的目的是开发和适应这种微流体技术，以经济有效的方式高通量制备DNA。

The sequencing of the human genome has stimulated the demands for DNA synthesis for hybridization, amplification and sequence analysis. Common DNA probes of primers are used once or twice, but typical methods for their synthesis prepares many thousand reactions. Orchid Biocomputer Inc. has prepared microfluidic chips and instrumentation for chemical synthesis. These devices allow minimal consumption of reagents for the preparation of 96 or 384 compounds from solid supports. It is the object of this grant to develop and adapt this microfluidic technology for the high throughput preparation of DNA in a cost-effective manner.