CASCADE ROLLING CIRCLE AMPLIFICATION FOR DNA DIAGNOSTICS

用于 DNA 诊断的级联滚环放大

基本信息

  • 批准号:
    6170748
  • 负责人:
  • 金额:
    $ 31.57万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    1997
  • 资助国家:
    美国
  • 起止时间:
    1997-09-30 至 2001-08-31
  • 项目状态:
    已结题

项目摘要

DESCRIPTION (Applicant's abstract): A novel isothermal DNA amplification technique has been developed called cascade rolling circle amplification (CRCA). This method is simple, rapid, and highly sensitive, achieving over a billion-fold amplification within one hour. By making one of the primers an energy transfer-labeled primer that generates a fluorescent signal only when incorporated into the amplicon, CRCA products can be detected within a closed system. These features make this technology suitable for a variety of diagnostic applications including both automated platforms and point-of-care field devices. Phase I research has demonstrated the feasibility of CRCA for DNA diagnostics, including detection of pathogenic agents. The objectives of this Phase II project include i) improvements in the assay to make the technology more adaptable to a variety of platforms, ii) development of multiplex assay using energy transfer-labeled primers of different colors, iii) optimizing the system for real-time detection to accurately quantitate target sequences, iv) adapting CRCA to a point-of-care diagnostic platform that is non-fluorescence based, and v) adapting the technology to an array platform. Long-range objectives include implementing CRCA in an automated amplification/detection system and developing broad applications for the point-of-care diagnostic market PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE
描述(申请人摘要):一种新的等温DNA扩增方法, 一种称为级联滚环放大的技术已经被开发出来 (CRCA)。该方法简便、快速、灵敏度高, 十亿倍的扩增通过使其中一种引物 能量转移标记的引物,其仅当 当CRCA产物被掺入扩增子中时,可以在封闭的扩增子内检测CRCA产物。 系统这些特性使该技术适用于各种 诊断应用,包括自动化平台和床旁诊断 现场设备第一阶段的研究已经证明了CRCA的可行性, DNA诊断,包括病原体检测。的目标 该第二阶段项目包括i)改进测定方法, 技术更适合各种平台,二)开发 使用不同颜色的能量转移标记引物的多重测定,iii) 优化实时检测系统,以准确定量靶标 iv)使CRCA适应床旁诊断平台, 基于非荧光的,以及v)使该技术适应阵列平台。 长期目标包括以自动化方式实施CRCA, 扩增/检测系统和开发广泛的应用, 床旁诊断市场 拟议商业应用:不可用

项目成果

期刊论文数量(0)
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会议论文数量(0)
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David C Thomas其他文献

Synthesis of 15N-Pyridines and Higher Mass Isotopologs via Zincke Imine Intermediates.
通过锌亚胺中间体合成 15N-吡啶和更高质量的同位素异构体。
  • DOI:
  • 发表时间:
    2024
  • 期刊:
  • 影响因子:
    15
  • 作者:
    Hillary M H Nguyen;David C Thomas;Marie A Hart;Kaila R Steenback;Jeffrey N. Levy;A. McNally
  • 通讯作者:
    A. McNally
Advancing interdisciplinary research: Insights from the JIBS special issue
  • DOI:
    10.1057/jibs.2014.34
  • 发表时间:
    2014-07-17
  • 期刊:
  • 影响因子:
    9.000
  • 作者:
    Joseph L C Cheng;Julian Birkinshaw;Donald R Lessard;David C Thomas
  • 通讯作者:
    David C Thomas
A novel mutation in EROS (CYBC1) causes Chronic Granulomatous Disease
EROS (CYBC1) 的新突变导致慢性肉芽肿病
  • DOI:
    10.1101/2023.04.21.23286144
  • 发表时间:
    2023
  • 期刊:
  • 影响因子:
    8.6
  • 作者:
    P. Mortimer;Esme Nichols;Joe Thomas;Rachna Shanbhag;Neha Singh;Eve L. Coomber;T. Malik;Matthew C. Pickering;Lyra O. Randzavola;Will M Rae;S. Bhattad;David C Thomas
  • 通讯作者:
    David C Thomas
From the Editors: Explaining theoretical relationships in international business research: Focusing on the arrows, NOT the boxes
  • DOI:
    10.1057/jibs.2011.44
  • 发表时间:
    2011-11-25
  • 期刊:
  • 影响因子:
    9.000
  • 作者:
    David C Thomas;Alvaro Cuervo-Cazurra;Mary Yoko Brannen
  • 通讯作者:
    Mary Yoko Brannen

David C Thomas的其他文献

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{{ truncateString('David C Thomas', 18)}}的其他基金

CASCADE ROLLING CIRCLE AMPLIFICATION FOR DNA DIAGNOSTICS
用于 DNA 诊断的级联滚环放大
  • 批准号:
    2792862
  • 财政年份:
    1997
  • 资助金额:
    $ 31.57万
  • 项目类别:
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