CHEMICAL SYNTHESIS OF RNA VIA NOVEL STRATEGY

描述：(改编自申请人的摘要)价值和需求 常规的rna寡核苷酸合成在过去一直在迅速增加。 十年来，研究揭示了RNA生物功能的范围。当前 方法能够以可接受的产量和质量合成RNA，但 似乎需要高水平的技能才能取得足够的成果。这个 NIH SBIR第二阶段项目的重点是实现强大的新RNA 科学界可以很容易地接触到合成技术。这个 将通过以下研究计划实现具体目标：核苷 5‘-和3’-试剂的化学优化及其在反应中的应用 其他实验室的合成方法及对经济型碱基的改进 大规模亚磷酰胺合成寡核苷酸合成化学 现行仪器的试剂、改装和方法的标准化 独立实验室联合优化2‘-ACE的测试与评估 化学在其他标准合成仪器上的成功实施 该研究计划将为通用合成仪器提供一个 合成高质量RNA的简便可靠的方法 寡核苷酸。利用一种现成的合成RNA寡核苷酸的方法，它 预计研究和与健康相关的发现将随之而来。 建议的商业应用：不可用

DESCRIPTION: (adapted from applicant's abstract) The value of and need for routine RNA oligonucleotide synthesis has been rapidly increasing over the past decade as research reveals the extent of RNA's biological functions. Current methods enable the synthesis of RNA in acceptable yields and quality, but a high level of skill appears to be required to deliver adequate results. The focus of this NIH SBIR Phase II project is to enable a powerful new RNA synthesis technology to be readily accessible to the scientific community. The specific aims will be achieved with the following research plan: Nucleoside chemistry Optimizations of 5'- and 3'-reagents and reactions Application of synthesis methods in other labs and to modified bases Development of economical large scale phosphoramidite synthesis Oligonucleotide synthesis chemistry Standardization of reagents, modifications and methods on current instrument Testing and evaluation in collaborating independent labs Optimization of 2'-ACE chemistry on other standard synthesis instruments The successful execution of this research plan will provide the general synthesis instruments with a readily available and reliable means of synthesizing high quality RNA oligonucleotides. With a ready means of synthesizing RNA oligonucleotides, it is expected that research and health-related discoveries will follow. PROPOSED COMMERCIAL APPLICTION: NOT AVAILABLE