WAVELET ENHANCEMENT OF CHROMOSOME BANDING PATTERNS

该项目旨在开发和商业化显着改进的软件，用于数字增强显微图像中染色体带型的细节。这些研究人员为这一应用开发了一种基于小波变换和多分辨率图像分析的创新技术。与现代计算机化的染色体分析结合使用，建议的技术有望显著改善染色体显带模式的增强，并更有效地对细微重排进行视觉检测。这将有助于临床医生和研究人员检测常规和高分辨率条带中以前不可见或亚可见的条带模式变化。它将显著提高自动化仪器的能力，以帮助评估临床样本以及正常和肿瘤哺乳动物细胞中的染色体变化。在第一阶段中，我们实现并测试了三个具有理想数学特性的小波变换。我们开发了一个用于染色体增强的多分辨率图像处理系统原型。我们获得了非常令人鼓舞的结果，有力地表明这些技术提供了比传统方法相当好的增强能力。并明确论证了该方法的可行性。在第二阶段，我们将完成软件的实施和完善。我们将实现几种小波设计方法，并评估许多小波变换基函数集，这些基函数集可能会在染色体带型模式中显示相关细节。拟议的商业应用：一旦新的增强技术被开发出来并符合常规应用的条件，它们将被纳入PSII的Powergene产品中，既包括在销售的新系统中，也包括作为对现有系统的升级。

This project aims to develop and commercialize significantly improved software for digital enhancement of the detail of chromosome banding patterns in microscopic images. These investigators have developed an innovative technique for this application, based upon wavelet transforms and multiresolution image analysis. Used with modern computerized chromosome analysis the proposed technique promises significantly improved enhancement of chromosome banding patterns and more effective visual detection of subtle rearrangements. This will help clinicians and researchers detect previously invisible or sub-visible band pattern alterations in conventional and high resolution banding. It will significantly increase the ability of automated instruments to assist the evaluation of chromosome alterations in clinical samples and in normal and neoplastic mammalian cells. During Phase I we implemented and tested three wavelet transforms with desirable mathematical properties. We developed a prototype multiresolution image processing system for chromosome enhancement. We obtained extremely encouraging results, strongly suggesting that these techniques offer considerably improved enhancement capability over conventional methods. and clearly demonstrating the feasibility of this approach. In Phase II we will complete the implementation and refinement of the software. We will implement several wavelet design approaches and evaluate many wavelet transform basis function sets that potentially can bring out relevant detail in chromosome banding patterns. PROPOSED COMMERCIAL APPLICATIONS: As soon as the new enhancement techniques are developed and qualified for routine application, they will be incorporated into PSII's PowerGene products, both in new systems sold and as an upgrade to existing systems.