BETA-CELL ANTIOXIDANT TRANSGENES IN DIABETES TRANSPLANT.

描述：（改编自研究者摘要）：胰腺β细胞 含有低水平的酶系统，保护免受活性氧 物种（ROS）。研究人员假设， 处理ROS是负责堡不寻常的敏感性的胰岛和 保护酶的增加将导致对1型和II型的抗性， 糖尿病和产生更有效的移植。来验证这些假设 研究人员已经培育出β细胞水平增加的转基因小鼠， 过氧化氢酶、金属硫蛋白和超氧化物歧化酶。在我们最初的 研究人员已经确定， 对β细胞功能是良性的。此外，过氧化氢酶 和金属硫蛋白对链脲佐菌素诱导的 糖尿病将测试转基因胰岛对额外β 细胞毒素，包括过氧化氢、白细胞介素1、一氧化氮和 过氧化物。这些转基因胰岛也将被测试用于改善 在同种异体受体的移植实验中的有效性。 由于ROS可能参与糖尿病的β细胞损伤，我们将测试 转基因动物是否能预防1型和II型糖尿病。 这些转基因小鼠提供了一个最佳的模型来测试ROS的作用， 糖尿病

DESCRIPTION: (Adapted from the Investigator's Abstract): Pancreatic beta-cells contain low levels of enzyme systems that protect against reactive oxygen species (ROS). The investigators hypothesize that the deficit in the ability to dispose of ROS is responsible fort the unusual sensitivity of the islet and that increasing protective enzymes will result in resistance to Type 1 and II diabetes and produce more effective transplantation. To test these hypotheses the investigators have produced transgenic mice with increased beta cell levels of catalase, metallothionein and superoxide dismutase. In our initial characterizations the investigators have determined that overexpression of each of these transgenes is benign to beta-cell function. In addition, both catalase and metallothionein provide dramatic protection against streptozotocin induced diabetes. Transgenic islets will be tested for resistance to additional beta cell toxins including hydrogen peroxide, interleukin 1, nitric oxide and superoxide. These transgenic islets will also be tested for improved effectiveness during transplantation experiments into allogenic recipients. Because ROS may be involved in beta cell damage in diabetes, we will test whether transgenic animals are protected from Type 1 and Type II diabetes. These transgenic mice provide an optimal model to test the role of ROS in diabetes.