BETA-CELL ANTIOXIDANT TRANSGENES IN DIABETES TRANSPLANT.

描述：(改编自《调查者摘要》)：胰岛β细胞 含有低水平的酶系统，可防止活性氧 种(ROS)。调查人员假设，这种能力的缺陷 ROS的处理对胰岛的异常敏感性负有责任 增加保护酶会导致对I型和II型的抵抗力 糖尿病和产生更有效的移植。为了检验这些假说 研究人员培育出了β细胞水平升高的转基因小鼠。 过氧化氢酶、金属硫蛋白和超氧化物歧化酶。在我们的初始阶段 研究人员已经确定了每种基因的过度表达 其中，转基因对β细胞功能是良性的。此外，两种过氧化氢酶 和金属硫蛋白对链脲佐菌素诱导的 糖尿病。转基因胰岛将接受对额外贝塔病毒的抗药性测试 细胞毒素，包括过氧化氢、白介素1、一氧化氮和 超氧化物。这些转基因胰岛也将进行改良测试。 同种异体受者移植实验期间的有效性。 由于ROS可能参与糖尿病的β细胞损伤，我们将测试 转基因动物是否受到1型和II型糖尿病的保护。 这些转基因小鼠提供了一种最佳的模型来测试ROS在 糖尿病。

DESCRIPTION: (Adapted from the Investigator's Abstract): Pancreatic beta-cells contain low levels of enzyme systems that protect against reactive oxygen species (ROS). The investigators hypothesize that the deficit in the ability to dispose of ROS is responsible fort the unusual sensitivity of the islet and that increasing protective enzymes will result in resistance to Type 1 and II diabetes and produce more effective transplantation. To test these hypotheses the investigators have produced transgenic mice with increased beta cell levels of catalase, metallothionein and superoxide dismutase. In our initial characterizations the investigators have determined that overexpression of each of these transgenes is benign to beta-cell function. In addition, both catalase and metallothionein provide dramatic protection against streptozotocin induced diabetes. Transgenic islets will be tested for resistance to additional beta cell toxins including hydrogen peroxide, interleukin 1, nitric oxide and superoxide. These transgenic islets will also be tested for improved effectiveness during transplantation experiments into allogenic recipients. Because ROS may be involved in beta cell damage in diabetes, we will test whether transgenic animals are protected from Type 1 and Type II diabetes. These transgenic mice provide an optimal model to test the role of ROS in diabetes.