CORE--PREPARATIVE AND MOLECULAR BIOLOGY

准备和数据管理核心将提供红细胞密度 高效液相色谱分离分析 为项目拨款提供分析和分子生物学支持。 大多数成员都使用一些准备和分析程序 在中心项目中。其中一些步骤(密度梯度 使用Percoll-Larex连续密度梯度进行分析)是 起源于这个实验室，并被证明在整理 细胞间异质性的后果是 镰状细胞综合征的特征(1-4)。程序 需要一名有经验的技术人员来准备 所需溶液(密度梯度混合物、高效液相色谱溶剂等) 执行有问题的分析，然后将值输入 供所有调查人员使用的实验室数据库。核心将会 还在准备用于扫描的样本方面提供建议和协助 透射电子显微镜。所用材料的费用和 目前使用的工具的服务合同是大量的 并且需要在项目之间共享。 红细胞密度分离分析在项目二、三、 六号和七号。 将在项目一、项目三和项目六中使用高效液相色谱来表征 珠蛋白链存在于人和小鼠的红细胞中。需要 在项目三和项目六中，分子生物学支持是不言而喻的。

The preparative and data management core will provide red cell density separation and analysis, HPLC (high performance liquid chromatography) analysis and molecular biology support to the program grant. A number of preparative and analytic procedures are used by most members of the center projects. Some of these procedures (density gradient analysis using Percoll-Larex continuous density gradients) were originated in this laboratory and have proved invaluable in sorting out the consequences of the intercellular heterogeneity which is characteristic of the sickle cell syndromes (1-4). The procedures require a portion of a technician who is experienced in preparing the needed solutions (density gradient mixes, HPLC solvents, etc.), performing the analysis in question, and then entering the values into the laboratory data base for use by all investigators. The core will also provide advice and assistance in preparing samples for scanning and transmission electron microscopy. Expenses for materials used and service contracts for the instruments currently utilized are substantial and need to be shared among the projects. Red cell density separation and analysis is used in Projects two, three, six and seven. HPLC will be used in projects one, three and six to characterize the globin chains present in human and mouse red cells. The need for molecular biology support is self explanatory in projects three and six.