CORE--PREPARATIVE AND MOLECULAR BIOLOGY

制备和数据管理核心将提供红细胞密度 分离和分析，HPLC（高效液相色谱） 分析和分子生物学支持的计划赠款。 大多数成员都使用一些准备和分析程序 的中心项目。 其中一些程序（密度梯度 使用Percol-Larex连续密度梯度分析）， 起源于这个实验室，并被证明是非常宝贵的， 细胞间异质性的后果， 镰状细胞综合征的特征（1-4）。 的程序 需要一部分有经验的技术人员来准备 所需溶液（密度梯度混合物、HPLC溶剂等）， 执行有问题的分析，然后将值输入到 实验室数据库供所有研究者使用。 芯会 还提供咨询和协助，准备样品进行扫描， 透射电子显微镜 所用材料和 目前使用的仪器的服务合同数额很大 并且需要在项目之间共享。 红细胞密度分离和分析用于项目二，三， 六号和七号。 HPLC将用于项目一、三和六，以表征 存在于人类和小鼠红细胞中的珠蛋白链。 需要 在项目三和项目六中，分子生物学支助是不言自明的。

The preparative and data management core will provide red cell density separation and analysis, HPLC (high performance liquid chromatography) analysis and molecular biology support to the program grant. A number of preparative and analytic procedures are used by most members of the center projects. Some of these procedures (density gradient analysis using Percoll-Larex continuous density gradients) were originated in this laboratory and have proved invaluable in sorting out the consequences of the intercellular heterogeneity which is characteristic of the sickle cell syndromes (1-4). The procedures require a portion of a technician who is experienced in preparing the needed solutions (density gradient mixes, HPLC solvents, etc.), performing the analysis in question, and then entering the values into the laboratory data base for use by all investigators. The core will also provide advice and assistance in preparing samples for scanning and transmission electron microscopy. Expenses for materials used and service contracts for the instruments currently utilized are substantial and need to be shared among the projects. Red cell density separation and analysis is used in Projects two, three, six and seven. HPLC will be used in projects one, three and six to characterize the globin chains present in human and mouse red cells. The need for molecular biology support is self explanatory in projects three and six.