MULTI MEGABASE SEQUENCING OF HUMAN CHROMOSOME 14

我们建议对人类14号染色体的选定区域进行测序，同时 开发生产线排序设施。我们计划给第三集排序 第一年到第三年分别为6Mb和12Mb的DNA。我们 将采用序列标记连接器(STC)策略来识别 细菌人工染色体克隆的最小平铺路径 跨越14号染色体。我们将使用随机或猎枪策略 对单个BAC克隆进行测序。我们创造的关键是， 整合和改进工厂生产线以进行排序将 成为我们的实验室信息管理系统(LIMS)。我们还计划 拥有一条模块化生产线，可以整合新战略或 用于大规模测序的仪器。

We propose to sequence selected regions of human chromosome 14 while developing a production line sequencing facility. We plan to sequence 3 Mb, 6Mb, and 12 Mb of DNA in years one through three, respectively. We will employ the sequence tagged connector (STC) strategy for identifying a minimum tiling path of bacterial artificial chromosome (BAC) clones across chromosome 14. We will use the random or shotgun strategy for sequencing the individual BAC clones. The key to our creating, integrating, and improving a factory production line for sequencing will be our laboratory information management system (LIMS). We also plan to have a modular production line that can incorporate new strategies or instrumentation for large-scale sequencing.