A STUDY OF THYMIC NURSE CELLS

胸腺护理细胞的研究

• 批准号: 6271629
• 负责人: JERRY GUYDEN
• 金额: $ 3.01万
• 依托单位: CITY COLLEGE OF NEW YORK
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-02-01 至 1999-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6271629
• 关键词: CD4 molecule; CD8 molecule; T lymphocyte; antibody specificity; cell line; ionizing radiation; laboratory mouse; laboratory rat; monoclonal antibody; organ culture; phagocytosis; phenotype; receptor binding; thymus

Thymic nurse cells (TNCs) are specialized epithelial components that contain T cells completely enclosed in intra-cytoplasmic vacuoles. Very little information about their function has been reported because of the lack of pure TNC populations in quantities necessary for their thorough analyses. However, they are believed to play a role in thymocyte development. Recently, our laboratory has been able to immortalize TNCs with SV40 virus. Fortunately, cells from these TNC lines have been shown to maintain thymocyte internalization capabilities in vitro. Further, we have shown that the only cells bound by and internalized into TNCs are CD4+ CD8+ thymocytes. These cell lines will be used to determine TNC function in the thymus. Two strategies will be employed: (1a) TNC- specific monoclonal antibodies (mAb) will be generated in an attempt to identify the receptor/ligand involved in binding and internalization. (b) These antibodies will be used to remove TNCs from the thymus in organ culture. A-lymphoid thymi treated with anti-TNC specific antibody will be recolonized with double positive thymocytes. The phenotype of the T cells recovered will be determined and compared to that of thymocytes isolated from identical thymi re-seeded with TNCs to pinpoint TNC function in thymocyte development. These antibodies will also be used to develop TNC-minus mice in vivo studies. (2) Antibody preparations shown to block binding of thymocytes to TNCs will be used to clone the gene(s) expressing the protein(s) involved. These studies are designed to determine the overall function of the thymocyte internalization by TNCs in the thymus.

胸腺哺育细胞(TNCs)是一种特殊的上皮成分， 含有完全封闭在胞质内空泡中的T细胞。非常 关于它们的功能的报道很少，因为 缺乏必要数量的纯跨国公司人口，以彻底 分析。然而，它们被认为在胸腺细胞中发挥作用。 发展。最近，我们的实验室已经能够让跨国公司永垂不朽 携带SV40病毒。幸运的是，来自这些TNC系的细胞已经显示出来 以维持胸腺细胞的体外内化能力。此外， 我们已经证明，唯一与跨国公司结合并内化到跨国公司中的细胞是 CD4+CD8+胸腺细胞。这些细胞系将被用来确定TNC 在胸腺中起作用。将采用两种战略：(1)跨国公司-- 将产生特定的单抗(MAb)，以尝试 确定参与结合和内化的受体/配体。 (B)这些抗体将被用来从器官中的胸腺中清除TNCs 文化。抗TNC特异性抗体治疗A淋巴样胸腺 用双阳性胸腺细胞重新克隆。T细胞的表型 回收的细胞将被确定并与胸腺细胞进行比较 从与TNCs重新接种的相同胸腺中分离出来以定位TNC 在胸腺细胞发育中的作用。这些抗体也将用于 开展TNC阴性小鼠的体内研究。(2)抗体制剂 阻断胸腺细胞与TNCs的结合将被用来克隆 表达涉及蛋白的基因(S)(S)。这些研究是专门设计的 通过测定胸腺细胞内化的整体功能 胸腺中的TNCs。