TRANSMISSION ELECTRON MICROSCOPE

此申请要求资助购买JOEL JEM-1010变速器 电子显微镜，数字图像采集系统，高分辨率 宾夕法尼亚大学医学中心（UPMC）和 电子显微镜核心设施是由该机构支持， 糖尿病和内分泌研究中心（DERC）和癌症中心。 该仪器将取代17年前安装的JOEL 100CX显微镜 在核心设施，已用于超微结构研究， 超过50名NIH支持的研究人员，发表了200多篇论文。 该申请确定了UPMC的17名NIH支持的研究人员， 主要用户群（Major User Group，MUG） 据估计，新增8- 每年12名项目规模较小的调查员也将受益 直接从购买该工具。 现有显微镜 实际使用时间几乎为每周40小时。 大量使用导致 某些机械系统的可预测和不可预防的恶化。 停机时间增加，变得不可接受，主要是因为 关键系统的替换部件，例如，摄像机机构，旋钮， 等等，必须被制造，重建，或从废弃的 仪器. 新仪器将由一位有经验的 MUG的专业和技术人员或由他们培训的其他人员。 该工具的利用和核心基金的运作情况如下： 由该机构的核心研究设施委员会和 咨询委员会，由监察组调查员和支助人员组成 中心. 这台新仪器在电子光学方面取得了进步， 数码相机系统将提高我们提供 向研究者传达最先进的超微结构分析， 以比商业公司更低的成本。 过去利用 核心贷款和本申请中提供的文件 证明该仪器将大大有利于NIH支持的 在这个机构和其他地方的研究。 应用程序支持 机构研究核心设施委员会，DERC和癌症 中心

This application requests funding to purchase a JOEL JEM-1010 transmission electron microscope, digital image acquisition system, and high resolution printer for the University of Pennsylvania Medical Center (UPMC) and the Electron Microscopy Core Facility which is supported by the Institution, the Diabetes and Endocrinology Research Center (DERC) and Cancer Center. The instrument will replace a JOEL 100CX microscope installed 17 years ago in the Core Facility which has been used for ultrastructural studies for over 50 NIH-supported investigators resulting in over 200 publications. This application identifies 17 NIH-supported investigators at UPMC and elsewhere as the Major User Group(MUG). It is estimated an additional 8- 12 investigators per year with less extensive projects also will benefit directly from the purchase of this instrument. The existing microscope is in actual use almost 40 hr per week. The heavy usage has resulted in predictable and unpreventable deterioration of some mechanical systems. Down-time has increased and become unacceptable, primarily because replacement parts for key systems, e.g., the camera mechanism, knobs, etc., have to be fabricated, rebuilt, or scavenged from discarded instruments. The new instrument will be operated by an experienced professional and technical staff for the MUG or others trained by them. Utilization of the instrument and the operational of the Core Facility are controlled by the Institution's Core Research Facilities Committee and an Advisory Committee composed of investigators from the MUG and supporting Centers. The new instrument, with its advancements in electron optics, and the digital camera system will enhance our ability to provide and communicate state-of-the-art ultrastructural analyses to investigators of the MUG at a lower cost than commercial firms. The past utilization of the Core Facility and the documentation provided in this application demonstrate this instrument will significantly benefit NIH-supported research at this Institution and elsewhere. The application is supported by the Institutional Research Core Facilities Committee, DERC, and Cancer Center.