ACQUISTION OF AN ELECTROSPRAY TANDEM MASS SPECTROMETER

购买电喷雾串联质谱仪

• 批准号: 2503084
• 负责人: JOHN A THOMPSON
• 金额: $ 17.08万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-15 至 1999-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2503084
• 关键词: biomedical equipment; biomedical equipment purchase; electrospray ionization mass spectrometry; gas chromatography mass spectrometry

In spite of the major advances in mass spectrometry during the past 10 years which have extended this technique to a wide variety of biological compounds with unparalleled sensitivity and specificity, and the fact that the University of Colorado Health Sciences Center (UCHSC) is a major research institution, there are no electrospray ionization (ESI), tandem quadrupole (MS-MS), or high resolution matrix assisted laser desorption instruments on this campus and none available to UCHSC investigators in the Denver area. In order to address this major shortcoming and to support several current NIH-funded projects, a group of six major users are requesting funds to purchase a versatile ESI MS-MS instrument which will fulfill current analytical needs and also serve to enhance the general awareness among UCHSC investigators that mass spectrometry can solve a wide variety of biochemical and biomedical problems. Administrations at UCHSC have provided excellent facilities and strong financial support for this acquisition to assure its efficient and long-term operation. The major use group consists of Drs. John A. Thompson, Paul V. Fennessey, Brad K. Bendiak, Charles S. McHenry, Roy B. Jones, Andrew S. Kraft, and James L. McManaman. Dr. Thompson will utilize the ESI MS-MS to investigate reactive metabolites of cytotoxic phenols, including electrophilic quinone methides which alkylate proteins and DNA, and peroxyquinols which oxidize methionine residues in proteins. Dr. Fennessey will utilize the instrument for ongoing studies on inborn errors in metabolism in pediatric subjects, specifically disorders results in elevated acylcarnitine levels. His group is also involved in protein and peptide analysis and those studies will be greatly facilitated by ESI MS-MS. Dr. Bendiak will be performing structural analyses of oligosaccharides released from human glycoproteins, and oligosaccharides that are sequentially degraded from the reducing end. Dr. McHenry will be examining recombinant subunit proteins of DNA polymerase and establish sites of co-or post-translational modification. He will also identify sites of interaction between subunits through studies with cross-linked peptides. Dr. Jones and Kraft will utilize ESP MS-MS to study the pharmacokinetics and metabolism of complex antineoplastic drugs in physiological fluids. Dr. McManaman will be examining the structures of peptides from aldehyde oxidase and xanthine oxidase to determine sites of post-translational modification and oxidative damage.

尽管过去 10 年来质谱技术取得了重大进展 多年来，该技术已扩展到多种生物领域 化合物具有无与伦比的敏感性和特异性，并且事实上 科罗拉多大学健康科学中心 (UCHSC) 是一个专业 研究机构，没有电喷雾电离（ESI）、串联 四极杆 (MS-MS) 或高分辨率基质辅助激光解吸 该校园内没有仪器可供 UHSC 调查人员使用 丹佛地区。为了解决这一重大缺陷并支持 目前 NIH 资助的几个项目中，有六个主要用户组 请求资金购买多功能 ESI MS-MS 仪器，该仪器将 满足当前的分析需求，也有助于增强总体分析能力 UHSC 研究人员意识到质谱可以解决 各种各样的生化和生物医学问题。行政部门在 UHSC 为我们提供了优良的设施和强有力的资金支持 此次收购旨在确保其高效、长期运营。这 主要使用组由博士组成。约翰·A·汤普森、保罗·V·芬尼西、布拉德 K. 本迪亚克、查尔斯 S. 麦克亨利、罗伊 B. 琼斯、安德鲁 S. 卡夫和詹姆斯 L.麦克马纳曼。 Thompson 博士将利用 ESI MS-MS 进行研究 细胞毒性酚的反应性代谢物，包括亲电子醌 烷基化蛋白质和 DNA 的方法，以及氧化的过氧喹啉 蛋白质中的蛋氨酸残基。 Fennessey 博士将使用该仪器 正在进行的关于儿科受试者先天性代谢缺陷的研究， 特别是疾病会导致酰基肉碱水平升高。他的组 还涉及蛋白质和肽分析，这些研究将 ESI MS-MS 极大地促进了这一过程。本迪亚克博士将表演 从人类糖蛋白释放的寡糖的结构分析， 以及从还原端依次降解的寡糖。 McHenry 博士将研究 DNA 重组亚基蛋白 聚合酶并建立共翻译或翻译后修饰位点。 他还将通过以下方式确定子单元之间相互作用的位点： 交联肽的研究。琼斯博士和卡夫将利用 ESP MS-MS 研究复合物的药代动力学和代谢 生理液中的抗肿瘤药物。麦克马纳曼博士将 检查醛氧化酶和黄嘌呤肽的结构 氧化酶以确定翻译后修饰位点和 氧化损伤。