ACQUISTION OF AN ELECTROSPRAY TANDEM MASS SPECTROMETER

尽管质谱学在过去10年中取得了重大进展 几年来，这项技术已经扩展到各种各样的生物学 具有无与伦比的敏感性和特异性的化合物，而事实是 科罗拉多大学健康科学中心(UCHSC)是一家 研究机构，没有电喷雾电离(ESI)，串联 四极杆(MS-MS)或高分辨率基质辅助激光解吸 在这个校园里有仪器，而UCHSC的调查人员在 丹佛地区。为了解决这一重大缺陷并支持 目前由美国国立卫生研究院资助的几个项目，一个由六个主要用户组成的小组是 申请资金购买多功能ESI MS-MS仪器，将 满足当前的分析需求，也有助于提高总体 UCHSC调查人员意识到质谱学可以解决 各种各样的生化和生物医学问题。行政部门在 UCHSC提供了一流的设施和强大的资金支持 此次收购以确保其高效和长期运营。这个 主要使用组包括John A.Thompson博士、Paul V.Fennessey博士和Brad 本迪亚克、查尔斯·S·麦克亨利、罗伊·B·琼斯、安德鲁·S·克拉夫特和詹姆斯 L.麦克马纳曼。汤普森博士将利用ESI MS-MS研究 细胞毒性酚的反应性代谢物，包括亲电性的苯二酚 使蛋白质和DNA烷化的甲基化合物，以及氧化的过氧喹酚 蛋白质中的蛋氨酸残留量。Fennessey博士将利用该仪器 对于正在进行的关于儿科受试者先天代谢错误的研究， 具体地说，疾病会导致酰肉碱水平升高。他的团队 还参与蛋白质和多肽分析，这些研究将是 由电喷雾质谱(ESI MS-MS)极大地促进。本迪亚克博士将表演 人糖蛋白释放的寡糖的结构分析， 以及从还原端依次降解的低聚糖。 麦克亨利博士将研究DNA的重组亚单位蛋白 聚合酶，并建立共翻译或翻译后修饰的位点。 他还将通过以下方式确定亚基之间的相互作用地点 交联肽的研究。琼斯博士和卡夫公司将利用ESP MS-MS法研究络合物的药代动力学和代谢 生理液体中的抗肿瘤药物。麦克马纳曼医生将成为 乙醛氧化酶和黄嘌呤多肽的结构研究 氧化物酶测定翻译后修饰位点和 氧化损伤。

In spite of the major advances in mass spectrometry during the past 10 years which have extended this technique to a wide variety of biological compounds with unparalleled sensitivity and specificity, and the fact that the University of Colorado Health Sciences Center (UCHSC) is a major research institution, there are no electrospray ionization (ESI), tandem quadrupole (MS-MS), or high resolution matrix assisted laser desorption instruments on this campus and none available to UCHSC investigators in the Denver area. In order to address this major shortcoming and to support several current NIH-funded projects, a group of six major users are requesting funds to purchase a versatile ESI MS-MS instrument which will fulfill current analytical needs and also serve to enhance the general awareness among UCHSC investigators that mass spectrometry can solve a wide variety of biochemical and biomedical problems. Administrations at UCHSC have provided excellent facilities and strong financial support for this acquisition to assure its efficient and long-term operation. The major use group consists of Drs. John A. Thompson, Paul V. Fennessey, Brad K. Bendiak, Charles S. McHenry, Roy B. Jones, Andrew S. Kraft, and James L. McManaman. Dr. Thompson will utilize the ESI MS-MS to investigate reactive metabolites of cytotoxic phenols, including electrophilic quinone methides which alkylate proteins and DNA, and peroxyquinols which oxidize methionine residues in proteins. Dr. Fennessey will utilize the instrument for ongoing studies on inborn errors in metabolism in pediatric subjects, specifically disorders results in elevated acylcarnitine levels. His group is also involved in protein and peptide analysis and those studies will be greatly facilitated by ESI MS-MS. Dr. Bendiak will be performing structural analyses of oligosaccharides released from human glycoproteins, and oligosaccharides that are sequentially degraded from the reducing end. Dr. McHenry will be examining recombinant subunit proteins of DNA polymerase and establish sites of co-or post-translational modification. He will also identify sites of interaction between subunits through studies with cross-linked peptides. Dr. Jones and Kraft will utilize ESP MS-MS to study the pharmacokinetics and metabolism of complex antineoplastic drugs in physiological fluids. Dr. McManaman will be examining the structures of peptides from aldehyde oxidase and xanthine oxidase to determine sites of post-translational modification and oxidative damage.