MICROMASS QUATTRO II MASS SPECTROMETER

MICROMASS QUATTRO II 质谱仪

• 批准号: 2503089
• 负责人: BILLY R MARTIN
• 金额: $ 37.26万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-15 至 1999-05-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2503089
• 关键词: biomedical equipment; biomedical equipment purchase; computer data analysis; computer program /software; mass spectrometry

The Mass Spectrometry Center at Virginia Commonwealth University currently consists of a Hewlett Packard 5988A quadrapole Mass Spectrometer (MS) that has been supported by the NIDA Center on Drug Abuse Research for the past eight years. We are requesting funds for the purchase of a MicroMass Quattro II MS in order to replace this instrument. The need for this replacement is two-fold: the Hewlett Packard 5988A has become outdated and it has limited capabilities. This proposal requests a multi-user instrument that will meet a wide range of MS needs within the university. Neuroscientists represent a major user group who are concentrating on drug abuse research. The primary thrust of their research has been the identification and quantitation of drugs in biological fluids where sensitivity is a major concern. Conversely, we have other researchers who are interested in the structural elucidation of carbohydrates, peptides and proteins where the availability of the state-of-the-art soft ionization capabilities is crucial. We have a third group of investigators who use direct probe for the structural verification of newly synthesized compounds. In order to accommodate the research needs of this wide range of scientists, we have chosen a Micromass Quattro II Triple Stage Quadropole Mass Spectrometer and the MassLynx-NT Mass Spectrometer Workstation. The Quattro II fulfills our needs because it has a multiple inlet adapter that accommodates direct probe, gas chromatography (GC) and liquid chromatography (LC) in contrast to almost to most instruments on the market that allow for either GC or LC, but not both. The Atmospheric Pressure Ionization LC-MS interface accommodates electrospray, nanospray, and atmospheric pressure chemical ionization that are particularly important for the structural elucidation of large molecules. Electron Ionization (EI) and Chemical Ionization (CI) capabilities are essential for full range of analyses by GC. The Direct Insertion Probe eliminates the need for development of either GC or LC methods for structural analysis of pure volatile samples. The HP 6890 GC will allow us to use many of the standard methods that have already been developed for quantitation of drugs biological fluids. The NIST library, BioLynx software, and the MaxEnt will provide us with reference spectra and allow us to sequence and determine molecular weights of proteins. We believe this requested instrumentation will allow these highly productive researchers to enhance their existing research programs as well as accommodate their needs for several years in the future.

弗吉尼亚联邦大学质谱中心 目前由Hewlett Packard 5988 A四极质量 光谱仪（MS）已由NIDA药物中心支持 过去八年的滥用研究。 我们正在申请资金 购买一台MicroMass Quattro II MS， 仪器 这种替代品的需求是双重的： Packard 5988 A已经过时，功能有限。 这 建议要求多用户仪器，将满足广泛的 大学里的MS需求。 神经科学家代表了一个主要的 专注于药物滥用研究的用户组。 主 他们研究的重点是识别和定量 敏感性是主要关注点的生物液体中的药物。 相反，我们有其他研究人员对 碳水化合物、肽和蛋白质的结构解析， 最先进的软电离能力的可用性， 至关重要的. 我们有第三组调查人员使用直接调查 用于新合成化合物的结构验证。 在 为了适应这种广泛的研究需求， 科学家们，我们选择了Micromass Quattro II三级 四极杆质谱仪和MassLynx-NT质谱仪 工作站 Quattro II满足了我们的需求，因为它具有 多入口适配器，可容纳直接探头、气体 气相色谱法（GC）和液相色谱法（LC），与几乎 市场上允许GC或LC的大多数工具，但 不能两者都有。 大气压电离LC-MS接口 可容纳电喷雾、纳米喷雾和大气压化学品 电离，这是特别重要的结构 大分子的解析。 电子电离（EI）和化学 电离（CI）能力对于全方位分析至关重要 通过GC。 直接插入式探头无需开发 GC或LC方法用于纯挥发性化合物的结构分析 样品 HP 6890 GC将允许我们使用许多标准的 已经开发的药物定量方法 生物液体 NIST文库、BioLynx软件和MaxEnt 将为我们提供参考光谱，并允许我们进行测序， 确定蛋白质的分子量。 我们认为这是 仪器将使这些高产的研究人员， 加强他们现有的研究计划，以及适应他们的 未来几年的需求。