PACEMAKER CURRENT AND ITS MODULATION IN YOUNG AND ADULT VENTRICLE

子项目摘要不可用。父摘要： 我们的节目重点关注心率和心律失常的决定因素 在年轻人身上。我们的假设是， 青少年的电生理功能受自主神经的影响 神经系统和心脏的电生理特性 这与成人有很大的不同，这需要我们深入研究 了解我们是否要预防和治疗心跳障碍。 因此，我们打算对新生、幼年和成年动物进行研究。我们的 方法是多方面的，因为电生理、生物物理 生化、药理学和细胞培养技术被用于 完整动物、分离组织、单细胞和亚细胞的研究 组件。尽管心律失常的发生通常可以被认为是 包括冲动启动和/或传导的异常，我们的 这五年的意图是专注于冲动激发。 以及它与复极化的关系。计划中的项目 重视对完整动物电生理特性的研究 分离的组织，脉冲启动的离子决定因素在 上述模型(方案A)以及单个心肌细胞和培养细胞，以及 受体-效应器耦合。 核心提供支持服务，以及细胞解聚和组织 文化。尽管我们的总体方法将成人作为主要的 参考人口，调查最密集的领域是 以新生儿和年轻人为中心。这些研究的意义在于 他们是面向年轻年龄组的，作为一个独特的群体 哪种节律和心律失常在它们的起源和自主神经上真正不同 来自成虫的调制。利用所获得的信息，我们不仅 将提高我们对负责正常的机制的理解 节奏和心律失常，但可以设计和寻找新的手段 心律失常的防治。

SUBPROJECT ABSTRACT NOT AVAILABLE. PARENT ABSTRACT: Our program focuses on the determinants of cardiac rhythm and arrhythmias in the young. Our hypothesis is that the expression of electrophysiologic function in the young is influenced by the autonomic nervous system and the electrophysiologic properties of the heart in ways that differ importantly from the adult, and that require our in depth understanding if we are to prevent and treat disorders of the heart beat. Hence, we intend to study the neonatal, young and adult animal. Our approach is multifaceted in that electrophysiological, biophysical, biochemical, pharmacological, and cell culture techniques are used in studies of intact animals, isolated tissues, single cells and subcellular components. Although arrhythmogenesis in general can be thought of as encompassing abnormalities of impulse initiation and/or conduction, our intent in this five-year period is to concentrate on impulse initiation and its relationship to repolarization. The projects in the Program stress the study of electrophysiologic properties of intact animals and isolated tissues, ionic determinants of impulse initiation studied in the above models (Project A) and in single myocytes and cultured cells, and receptor-effector coupling. The Cores provide support services, and cell disaggregation and tissue culture. Although our overall approach includes the adult as a major reference population, the most intensive area of investigation is centered on the neonate and young. The significance of these studies is that they are geared to the young age group as a unique population in which rhythm and arrhythmias truly differ in their genesis and autonomic modulation from the adult. Using the information obtained we not only shall improve our understanding of the mechanisms responsible for normal rhythm and arrhythmias but can design and seek new means for the prevention and treatment of arrhythmias.