CORE--CELL ISOLATION AND CULTURE

细胞分离和培养核心提供专业知识和服务， 所有实验的设计、执行和解释， 使用培养的细胞和细胞系。 核心人员有 在细胞培养领域的经验。 的具体目标 核心是1）分离和培养所需的原代细胞和细胞系 由本申请中提出的五个项目的每个研究员， 2)维持特殊的细胞系（例如，稳定转染气管 肌细胞），3）验证质量 拟定中使用的所有细胞培养物和细胞系的对照 （4）维护调查所需的设施和设备; 项目，5）根据需要开发细胞培养新技术， 每个项目，并指导新的项目研究人员在适当的 法 该中心提供的具体服务包括： 特异性原代细胞和细胞分离、生长和制备 线路，咨询实验设计，提供和维护 使用提供的质粒转染细胞系 调查人员在具体项目中的工作，并提供所需的数据 以验证细胞系和转染的细胞。 一流设施和 参与这一核心的人员在每一个科学领域都发挥着作用， 项目 每个项目都需要隔离和/或培养 用于实验的细胞。 有一些常见的技术， 更有效的规模经济。 此外，通用设备需要 对于细胞的长期储存和细胞转染， 通过核心设施。 最后，我们有必要 可用于应对和适应新方向的经验和资源 的调查。 所有项目都将受益于与此核心的互动 设施。

The Cell Isolation and Culture Core provides expertise and services in the design, performance and interpretation of all experiments in which cultured cells and cell lines are used. Personnel in the core have experience spanning the field of cell culture. The specific aims of the core are to 1) isolate and culture primary cells and cell lines required by each investigator for the five projects proposed in this application, 2) maintain special cell lines (e.g., stably-transfected tracheal myocytes) required for proposed investigations, 3) validate quality control for all cell cultures and cell lines used in proposed investigations, 4) maintain facilities and equipment required for these projects, and 5) develop new techniques in cell culture as required for each project, and instruct new project research personnel in proper technique. The specific services to be provided by this core are: isolation, growth and preparation of specific primary cells and cell lines, consultation on experimental design, provision and maintenance of specialized cell lines, transfection of cell lines using plasmids provided by investigators in the specific projects, and provision of data required to validate cell lines and transfected cells. The facilities and personnel involved in this core have a role in each of the scientific projects. Each project shares a need for isolation and/or culture of cells for use in experiments. There are common techniques that will have a more efficient economy of scale. In addition, common equipment needs for long-term storage of cells, and cell transfections, can be met more easily through the Core Facility. Finally, we have the necessary experience and resources available to respond and adapt to new directions of inquiry. All projects will benefit from the interaction with this Core Facility.