STRUCTURAL AND ELEMENTAL ANALYSIS OF MACROMOLECULAR ASSEMBLIES
STRUCTURAL AND ELEMENTAL ANALYSIS OF MACROMOLECULAR ASSEMBLIES
批准号:
6111885
负责人:
S BRIAN Andrews
金额:
$0.0万
依托单位国家:
美国
项目类别:
财政年份:
--
资助国家:
美国
项目状态:
未结题
起止时间:
至
关键词:
Alphaherpesvirinae alternatives to animals in research axon cytoskeleton intermolecular interaction macromolecule microtubules molecular shape molecular weight neurofilament neuronal transport phosphorus phosphorylation protein sequence protein structure function scanning transmission electron microscopy spectrometry squid structural biology
中文摘要
这个项目的目标是描述形状的特征,
聚合物的分子量分布和元素组成
单个大分子和大分子组装体,具有
强调钙结合和调节蛋白的磷酸化
蛋白质。该项目利用了场发射技术的优势。
带电子扫描电子显微镜(STEM)
能量损失谱(EELS)?一种名为
光谱成像?分析并绘制…的分布图
生理上重要的元素,如磷和钙,分辨率为
优于10 nm。这项技术的应用如下所示
去极化状态下外周线粒体的光谱成像
蛙交感神经节神经元。线粒体内钙图谱
显示钙积聚的焦点位置小于10 nm
直径;这些部位内的钙浓度足够高
即使是单个像素的光谱也显示出显著的情况
123号鳗鱼边。这一信号的量化与
空间平均总钙浓度为~10 mmol/kg干重。
这些结果首次显示了一个定量的元素
单个线粒体内的分布图。战略以实现
用EELS说明了高分辨率的单原子探测
包括烟草花叶病毒在内的一系列标本的作图
(TMV)、分离的DNA质粒和磷酸化蛋白。
TMV的光谱成像产生了对应于1-3P的P图
病毒粒子投射中心区域的每像素原子
RNA链所在的位置,而在中心区之外
P原子的数目为零。结果表明,单一的
磷原子在生物组装中是可以检测到的。
英文摘要
The goal of this project is to characterize the shape,
molecular weight distribution and elemental composition of
individual macromolecules and macromolecular assemblies, with
emphasis on calcium binding by, and phosphorylation of, regulatory
proteins. This project takes advantage of combining field-emission
scanning transmission electron microscopy (STEM) with electron
energy loss spectroscopy (EELS) ? a new technique known as
spectrum imaging ? to analyze and map the distribution of
physiologically important elements like P and Ca at a resolution of
better than 10 nm. Application of this technology is illustrated by
spectrum imaging of peripheral mitochondria in depolarized
neurons of frog sympathetic ganglia. Intramitochondrial Ca maps
reveal focal sites of Ca accumulation that are less than 10 nm in
diameter; the Ca concentration within these sites is sufficiently high
that a spectrum from even a single pixel exhibits a prominent Ca
L23 EELS edge. Quantitation of this signal is consistent with a
spatially averaged total Ca concentration of ~10 mmol/kg dry wt.
These results show for the first time a quantitative elemental
distribution map within an individual mitochondrion. Strategies for
single atom detection at high resolution are illustrated by EELS
mapping of a series of specimens including tobacco mosaic virus
(TMV), isolated DNA plasmids, and phosphorylated proteins.
Spectrum imaging of TMV yields P maps that correspond to 1-3 P
atoms per pixel in the projected central region of the viral particles
where the RNA strand lies, whereas outside the central region the
number of P atoms is zero. The results demonstrate that single
atoms of phosphorus are detectable within biological assemblies.
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Elemental And Structural Organization Of Neurons And Gli
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批准号:6671356
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:S BRIAN Andrews
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依托单位:
ELEMENTAL AND STRUCTURAL ORGANIZATION OF NEURONS AND GLIA
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批准号:6163016
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:S BRIAN Andrews
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依托单位:
ELEMENTAL AND STRUCTURAL ORGANIZATION OF NEURONS AND GLIA
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批准号:6111844
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:S BRIAN Andrews
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依托单位:
Elemental And Structural Organization Of Neurons And Glia
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批准号:8746767
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项目类别:
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资助金额:$92.53万
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财政年份:--
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负责人:S BRIAN Andrews
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依托单位:
Elemental And Structural Organization Of Neurons And Gli
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批准号:7143827
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:S BRIAN Andrews
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依托单位:
Elemental And Structural Organization Of Neurons And Glia
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批准号:7735253
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项目类别:
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资助金额:$138.67万
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财政年份:--
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负责人:S BRIAN Andrews
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依托单位:
STRUCTURAL AND ELEMENTAL ANALYSIS OF MACROMOLECULAR ASSEMBLIES
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批准号:2579609
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项目类别:
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资助金额:$0.0万
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负责人:S BRIAN Andrews
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依托单位:
ELEMENTAL AND STRUCTURAL ORGANIZATION OF NEURONS AND GLIA
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批准号:6432892
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项目类别:
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资助金额:$0.0万
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财政年份:--
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负责人:S BRIAN Andrews
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依托单位:
Elemental And Structural Organization Of Neurons And Glia
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批准号:8342197
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项目类别:
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资助金额:$129.93万
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财政年份:--
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负责人:S BRIAN Andrews
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依托单位:
Elemental And Structural Organization Of Neurons And Glia
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批准号:8557000
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项目类别:
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资助金额:$99.95万
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负责人:S BRIAN Andrews
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依托单位:
Elemental & Structural Organization Of Neurons And Glia
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批准号:6842425
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资助金额:$0.0万
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负责人:S BRIAN Andrews
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依托单位:
ELEMENTAL AND STRUCTURAL ORGANIZATION OF NEURONS AND GLIA
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批准号:6290629
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资助金额:$0.0万
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负责人:S BRIAN Andrews
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依托单位:
Elemental And Structural Organization Of Neurons And Glia
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批准号:8149626
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项目类别:
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资助金额:$133.97万
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财政年份:--
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负责人:S BRIAN Andrews
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依托单位:
ELEMENTAL AND STRUCTURAL ORGANIZATION OF NEURONS AND GLIA
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批准号:2579549
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项目类别:
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资助金额:$0.0万
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负责人:S BRIAN Andrews
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依托单位:
Elemental And Structural Organization Of Neurons And Gli
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批准号:7323014
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资助金额:$0.0万
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负责人:S BRIAN Andrews
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依托单位:
Elemental And Structural Organization Of Neurons And Gli
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批准号:6990004
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资助金额:$0.0万
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负责人:S BRIAN Andrews
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依托单位:
Elemental And Structural Organization Of Neurons And Gli
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批准号:6507472
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资助金额:$0.0万
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负责人:S BRIAN Andrews
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依托单位:
Elemental And Structural Organization Of Neurons And Glia
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批准号:7594650
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资助金额:$142.57万
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负责人:S BRIAN Andrews
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依托单位:
Elemental And Structural Organization Of Neurons And Glia
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批准号:7969522
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项目类别:
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资助金额:$116.05万
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负责人:S BRIAN Andrews
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依托单位:
STRUCTURAL AND ELEMENTAL ANALYSIS OF MACROMOLECULAR ASSEMBLIES
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批准号:6163056
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项目类别:
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资助金额:$0.0万
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负责人:S BRIAN Andrews
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