SIMIAN D RETROVIRUSES 4 & 5 SEQUENCE ANALYSES & IMPLICATIONS FOR DIVERGENCE

去年我们报道了D4/Cyn/CA的分子克隆和 D5/HRE/或。我们现在描述推导的氨基酸序列，该序列由 完整的D5/RHE/OR基因组和D4/Cyn/CA环境基因序列 糖蛋白。 D5/RHE/OR基因组包含典型的D型基因 结构(5‘ltr-gag-prt-pol-env-3’ltr)，并对截断的8045进行编码 BP前病毒。5‘LTR全长329个核苷酸，具有典型的 整合、复制和表达的逆转录病毒决定因素， 包括U3、R和U5区域，候选Tata Box和CAT 序列，以及潜在的多聚腺苷信号。有趣的是， 与所有其他分子克隆的SRV不同，D5/RHE/OR 多聚腺苷酸化信号与大多数 真核基因和其他病毒中的基因。D5/RHE/OR gag基因编码 一种由660个氨基酸组成的多蛋白。就像在所有SRV中一样，Gag多聚蛋白 含有加工过的Gag分子p10、pp24/pp18、p12、p27、p14和 P4.D5/RHE/OR病毒蛋白水解酶全长314个氨基酸，含有 一个常见的Asp-Thr-Gly基序；PrT基因与Gag基因重叠61% 由8个残基组成的pol1基因。D5/RHE/或POL基因 编码一个由864个氨基酸组成的产物，比 其他SRV的POL产品。D5/RHE/OR env多聚蛋白为585 氨基酸残基长(gp70和gp20分子分别为394和191 氨基酸残基)，含有12个天冬氨酸-X-丝氨酸(Thr) 糖基化位点，其中11个位于gp70内。D4/CYN/CA 包膜蛋白全长582个氨基酸(gp70和gp20分子 分别为390个和192个残基)。D4/Cyn/CA gp70和gp20 分子为61.8-68.7%(范围)和84.9-87.5%(范围)，类似于 在所有其他SRV中确定相应的包膜糖蛋白 血清组。D4/Cyn/CA和D5/RHE/OR序列的比较 所有其他分子克隆的SRV都提供了有价值的见解 关于D型猿猴逆转录病毒的进化分化 一家人。

Last year we reported the molecular cloning of D4/CYN/CA and D5/HRE/OR. We now describe the deduced amino acid sequence encoded by the complete D5/RHE/OR genome, and the sequence of the D4/CYN/CA env glycoprotein. The D5/RHE/OR genome contains the prototypical type D genetic structure (5'LTR-gag-prt-pol-env-3'LTR) and encodes a truncated 8045 bp provirus. The 5' LTR is 329 bp in length and possesses typical retroviral determinants of integration, replication and expression, including the U3, R and U5 regions, candidate TATA box and CAT sequences, and the potential polyadenylation signal. Interestingly, unlike all other molecularly-cloned SRVs, the D5/RHE/OR polyadenylation signal is identical to the signal found in most eukaryotic genes and in other viruses. The D5/RHE/OR gag gene encodes a polyprotein of 660 amino acids. As in all SRVs, the gag polyprotein contains the processed gag molecules p10, pp24/pp18, p12, p27, p14 and p4. The D5/RHE/OR viral protease is 314 amino acids long and contains a common Asp-Thr-Gly motif; the prt gene overlaps the gag gene by 61 residues, and the pol gene by 8 residues. The D5/RHE/OR pol gene encodes a product of 864 amino acids, which is 3 residues shorter than the pol products of other SRVs. The D5/RHE/OR env polyprotein is 585 amino acid residues long (gp70 and gp20 molecules are 394 and 191 amino acid residues, respectively), and contains 12 Asn-X-Ser(Thr) glycosylation sites, 11 of which are found within gp70. The D4/CYN/CA envelope polyprotein is 582 amino acids long (gp70 and gp20 molecules are 390 and 192 residues, respectively). The D4/CYN/CA gp70 and gp20 molecules are 61.8-68.7% (range) and 84.9-87.5% (range), similar to corresponding env glycoproteins identified for all other SRV serogroups. The comparison of D4/CYN/CA and D5/RHE/OR sequences to all other molecularly-cloned SRVs has provided valuable insight concerning the evolutionary divergence of the type D simian retrovirus family.