NEW METHODS OF RNA PROTEIN INTERACTION STUDY: REV BINDING, HIV TAR RNA 3D STRUCT

作为理解RNA-蛋白质相互作用的模型，我们 研究Rev反应元件RNA的结构， 来源于Rev蛋白的肽。 Rev是两个小的基本 HIV中的调节蛋白。 将Rev绑定到Rev-response元素 RNA导致剪接D和未剪接病毒的比例发生变化 出现在细胞质中的mRNA。 这种相互作用的形成是 是艾滋病毒复制周期的关键点 此外我们还 检查HIV TAR RNA与烟酰胺复合的模型， 在上茎中的A-U碱基对之间形成的碱基三联体 HIV TAR RNA。

As a model for understanding RNA-protein interactions, we are studying the structure of the Rev Response Element RNA in complex with peptides derived from the Rev protein. Rev is one of two small basic regulatory protein in HIV. Binding of Rev to the Rev-response element RNA results in a change in the ratio of spliceD and unspliced viral mRNAs that appear in the cytoplasm. Formation of this interaction is a key point in the replication cycle of HIV. In addition, we are also examining a model of the HIV TAR RNA in complex with argininamide and the base triple formed between an A-U base pair in the upper stem of HIV TAR RNA.