BINDING OF COPPER(II) TO PULMONARY ENDOTHELIAL CELLS

铜 (II) 与肺内皮细胞的结合

• 批准号: 6118843
• 负责人: MARILYN P MERKER
• 金额: $ 0.06万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6118843
• 关键词: bioengineering /biomedical engineering; biological products; biomedical resource; enzymes; proteins

ESR methods were used to detect cupric ion on pulmonary arterial endothelial cells grown to confluence on gelatin-coated Biosilon beads (see highlight). Upon addition of 150 micro molar Cu(II) to these cells, EPR spectra were obtained from cells and supernate after various times of incubation with Cu(II) and in the presence and absence of bathocuprione, a colorimetric agent for Cu(I). An EPR signal for a type 2 Cu(II) site with g(parallel)=2.18 and A(parallel)=180G is attributed to cupric ion bound to cells. Addition of bathocuprione not only formed a complex with Cu(I), but also with Cu(II) with g(parallel)=2.19 and A(parallel)=160G. Additional studies are planned to determine the number of nitrogens bound to the Cu(II) on cells using low frequency, S-band, EPR methods. Further work is planned to help understand the significance of this site with respect to trans-membrane electron transport.

用ESR法检测肺动脉血中铜离子浓度 内皮细胞在明胶包被的生物素微珠上生长至汇合 (see突出显示）。 在向这些溶液中加入150微摩尔的Cu（II）后， 细胞，EPR谱从细胞和上清液中获得， 与Cu（II）孵育的不同时间， 不存在浴铜氢酮，一种用于Cu（I）的比色剂。 的EPR 2型Cu（II）位点的信号，g（平行）=2.18， A（平行）= 180 G归因于与细胞结合的铜离子。 此外 的bathocuprione不仅与Cu（I）形成络合物，而且与 Cu（II），g（平行）=2.19，A（平行）= 160 G。 其他研究 计划确定与Cu（II）结合的氮的数量， 用低频S波段EPR方法检测细胞 进一步的工作 计划帮助理解这个网站的重要性， 到跨膜电子传递。