PARAMAGNETIC COMPLEXES IN ALLOGENIC HETEROTROPIC CARDIAC TRANSPLANTS

同种异体异向性心脏移植中的顺磁性复合物

• 批准号: 6118848
• 负责人: GALEN M PIEPER
• 金额: $ 0.15万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6118848
• 关键词: bioengineering /biomedical engineering; biological products; biomedical resource; enzymes; proteins

We showed direct evidence using ESR analysis of the sequestration of heme-nitrosyl complexes within cardiac allografts prior to rejection using an improved tissue processing technique. We showed that heme-nitrosyl signals consistent with nitrosylmyoglobin is a prominent feature which is specific for tissue prior to rejection of cardiac allografts and not seen in isografts. This nitrosylmyoglobin signal in allografts is decreased by prior treatment of recipients with the antioxidant PDTC. As myoglobin is important to releasing oxygen for normal cardiac respiration and function, further studies will be necessary to evaluate whether decreases in nitrosylation of myoglobin contribute directly to amelioration of myocardial contractile dysfunction in cardiac allografts and to improvement in graft survival.

我们通过ESR分析发现了 血红素-亚硝酰复合物在心脏同种异体移植物中的含量 使用改进的组织处理技术进行排斥。 我们展示 血红素-亚硝基信号与亚硝基肌红蛋白一致， 在排斥反应发生前对组织特异性的突出特征 同种异体心脏移植物中未见。 这种亚硝基肌红蛋白 受体的预先治疗降低了同种异体移植物中的信号 抗氧化剂PDTC 由于肌红蛋白对于释放 氧气用于正常心脏呼吸和功能，进一步研究 将有必要评估是否减少亚硝基化的 肌红蛋白直接有助于改善心肌 心脏同种异体移植物中的收缩功能障碍和改善 移植物存活率