PARAMAGNETIC COMPLEXES IN ALLOGENIC HETEROTROPIC CARDIAC TRANSPLANTS

同种异体异向性心脏移植中的顺磁性复合物

• 批准号: 6118848
• 负责人: GALEN M PIEPER
• 金额: $ 0.15万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6118848
• 关键词: bioengineering /biomedical engineering; biological products; biomedical resource; enzymes; proteins

We showed direct evidence using ESR analysis of the sequestration of heme-nitrosyl complexes within cardiac allografts prior to rejection using an improved tissue processing technique. We showed that heme-nitrosyl signals consistent with nitrosylmyoglobin is a prominent feature which is specific for tissue prior to rejection of cardiac allografts and not seen in isografts. This nitrosylmyoglobin signal in allografts is decreased by prior treatment of recipients with the antioxidant PDTC. As myoglobin is important to releasing oxygen for normal cardiac respiration and function, further studies will be necessary to evaluate whether decreases in nitrosylation of myoglobin contribute directly to amelioration of myocardial contractile dysfunction in cardiac allografts and to improvement in graft survival.

我们通过ESR分析发现了直接的证据