PARAMAGNETIC COMPLEXES IN ALLOGENIC HETEROTROPIC CARDIAC TRANSPLANTS

同种异体异向性心脏移植中的顺磁性复合物

• 批准号: 6118848
• 负责人: GALEN M PIEPER
• 金额: $ 0.15万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-03-01 至 2000-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6118848
• 关键词: bioengineering /biomedical engineering; biological products; biomedical resource; enzymes; proteins

We showed direct evidence using ESR analysis of the sequestration of heme-nitrosyl complexes within cardiac allografts prior to rejection using an improved tissue processing technique. We showed that heme-nitrosyl signals consistent with nitrosylmyoglobin is a prominent feature which is specific for tissue prior to rejection of cardiac allografts and not seen in isografts. This nitrosylmyoglobin signal in allografts is decreased by prior treatment of recipients with the antioxidant PDTC. As myoglobin is important to releasing oxygen for normal cardiac respiration and function, further studies will be necessary to evaluate whether decreases in nitrosylation of myoglobin contribute directly to amelioration of myocardial contractile dysfunction in cardiac allografts and to improvement in graft survival.

我们使用封存的 ESR 分析提供了直接证据 心脏同种异体移植物中的血红素-亚硝酰复合物 使用改进的组织处理技术进行排斥。 我们展示了 与亚硝酰肌红蛋白一致的血红素亚硝酰信号是 排斥前组织特有的显着特征 心脏同种异体移植物，在同种移植物中未见。 这种亚硝基肌红蛋白 同种异体移植物中的信号因接受者的先前治疗而减弱 与抗氧化剂PDTC。 由于肌红蛋白对于释放很重要 正常心脏呼吸和功能的氧气，进一步研究 将有必要评估亚硝基化是否减少 肌红蛋白直接促进心肌的改善 同种异体心脏移植物的收缩功能障碍和改善 移植物存活。