PARAMAGNETIC COMPLEXES IN ALLOGENIC HETEROTROPIC CARDIAC TRANSPLANTS

我们用电子自旋共振分析提供了直接证据。 同种异体心脏移植物内的亚硝基-血红素复合体 使用改进的组织处理技术进行排斥反应。我们展示了 与亚硝酸肌红蛋白一致的亚硝基信号是一种 排斥反应前组织特有的显著特征 同种异体心脏移植，同种异体心脏移植物中未见。这种亚硝酸肌红蛋白 受体的预先治疗使同种异体移植物中的信号减少 使用抗氧化剂PDTC。因为肌红蛋白对释放 氧对正常心脏呼吸和功能的进一步研究 将有必要评估是否减少了亚硝化 肌红蛋白对改善心肌梗死有直接作用 同种异体心脏移植物的收缩功能障碍及其改善 移植物存活率。

We showed direct evidence using ESR analysis of the sequestration of heme-nitrosyl complexes within cardiac allografts prior to rejection using an improved tissue processing technique. We showed that heme-nitrosyl signals consistent with nitrosylmyoglobin is a prominent feature which is specific for tissue prior to rejection of cardiac allografts and not seen in isografts. This nitrosylmyoglobin signal in allografts is decreased by prior treatment of recipients with the antioxidant PDTC. As myoglobin is important to releasing oxygen for normal cardiac respiration and function, further studies will be necessary to evaluate whether decreases in nitrosylation of myoglobin contribute directly to amelioration of myocardial contractile dysfunction in cardiac allografts and to improvement in graft survival.