REGULATION OF CYTIDYLYLTRANSFERASE ACTIVITY

该实验室的工作主要集中在哺乳动物磷脂酰胆碱代谢的调节上，该代谢受胆碱激酶和CTP：胆碱磷酸胞苷转移酶(CCT)的调节。我们假设，细胞胆碱激酶活性受某些细胞中胆碱激酶同工酶的选择性表达和对某些信号的反应的调节。我们正在分离、表达和分析哺乳动物和线虫的胆碱激酶亚型的表达模式。此外，我们正在研究磷酸化和脂类对CCT活性的调节，并一直在研究该酶的催化机理。我们使用重组DNA技术进行所有这些研究，并需要使用GCG数据库和序列分析软件来继续进行这些研究。

Work in this laboratory has focused on the regulation of mammalian phosphatidylcholine metabolism, which is regulated by the enzymes choline kinase and CTP:cholinephosphate cytidylyltransferase (CCT). We hypothesize that cellular choline kinase activity is modulated by the selective expression of choline kinase isozymes in certain cells and in response to certain signals. We are isolating, expressing, and analyzing the expression patterns of choline kinase isoforms from mammals and the nematode, C. elegans. In addition, we are studying the regulation of CCT activity by phosphorylation and lipids, and have been studying the catalytic mechanism of this enzyme. We use recombinant DNA techniques for all these studies, and require the use of the GCG data base and sequence analysis software to continue them.