The lung in HIV disease

该项目的总体目标是研究来自HIV感染患者的肺泡巨噬细胞的功能，量化这些细胞中的HIV水平，并研究HIV在肺泡巨噬细胞中复制的决定因素以及HIV感染巨噬细胞功能后果的潜在机制。在GCRC上进行支气管镜检查以获得本研究中使用的细胞。一项亚研究是在hiv感染者的纵向研究中，通过检查支气管肺泡细胞与血液单核细胞的表型和病毒载量，以及可能影响LAL发病机制的选定功能，来定义淋巴细胞性肺泡炎（LAL）的自然史。在这项对20名无肺部疾病史的无症状hiv感染者的年度纵向分析中，受试者有2次访问GCRC：一次进行问卷调查、CXR和PFTs，第二次在1或2周后进行灌洗和静脉穿刺。正在进行的艾滋病毒感染者与健康对照的横断面研究将继续进行。

The overall goals of this project are to examine the functions of alveolar macrophages from a spectrum of HIV infected patients and to quantitate the level of HIV in these cells and to examine determinants of replication of HIV in alveolar macrophages and the mechanisms underlying the functional consequences of HIV infection in macrophages. Bronchoscopies are performed on the GCRC to obtain cells used in this study. One substudy is to define the natural history of lymphocytic alveolitis (LAL) in a longitudinal study of HIV-infected subjects by examining the phenotype and viral load in bronchoalveolar cells versus blood mononuclear cells and selected functions that could impact on the pathogenesis of LAL. In this longitudinal yearly analysis of 20 asymptomatic HIV-infected subjects with no previous history of pulmonary disease, subjects have 2 visits to the GCRC: one for questionnaire, CXR, and PFTs and the second at 1 or 2 weeks later for lavage and venipuncture. Ongoing cross-sectional studies of HIV infected versus healthy controls will continue.