The lung in HIV disease

该项目的总体目标是检查一系列艾滋病毒感染患者的肺泡巨噬细胞的功能，量化这些细胞中艾滋病毒的水平，并检查艾滋病毒在肺泡巨噬细胞中复制的决定因素和艾滋病毒感染巨噬细胞中潜在的功能后果的机制。在GCRC上进行支气管镜检查以获得本研究中使用的细胞。其中一项子研究是在一项针对HIV感染者的纵向研究中，通过检测支气管肺泡细胞与血液单个核细胞的表型和病毒载量以及可能影响淋巴细胞性肺泡炎发病机制的选定功能，来确定淋巴细胞性肺泡炎(LAL)的自然病史。在这项对20名没有肺部疾病病史的无症状HIV感染者的年度纵向分析中，受试者有两次去GCRC：一次是为了问卷、CXR和PFT，第二次是在一到两周后进行洗涤和静脉穿刺术。目前正在进行的艾滋病毒感染与健康对照的横断面研究将继续进行。

The overall goals of this project are to examine the functions of alveolar macrophages from a spectrum of HIV infected patients and to quantitate the level of HIV in these cells and to examine determinants of replication of HIV in alveolar macrophages and the mechanisms underlying the functional consequences of HIV infection in macrophages. Bronchoscopies are performed on the GCRC to obtain cells used in this study. One substudy is to define the natural history of lymphocytic alveolitis (LAL) in a longitudinal study of HIV-infected subjects by examining the phenotype and viral load in bronchoalveolar cells versus blood mononuclear cells and selected functions that could impact on the pathogenesis of LAL. In this longitudinal yearly analysis of 20 asymptomatic HIV-infected subjects with no previous history of pulmonary disease, subjects have 2 visits to the GCRC: one for questionnaire, CXR, and PFTs and the second at 1 or 2 weeks later for lavage and venipuncture. Ongoing cross-sectional studies of HIV infected versus healthy controls will continue.