BLOOD FLOW IN MURINE SKELETAL MUSCLE: GLUCOSE METAB VIA SORBITOL PATHWAY

这项研究的目的是评估糖代谢的作用。 通过山梨醇途径调节心肌血流量增加 转基因小鼠(6-8wk.指年龄)过度表达骨骼肌 过剩-1。评估比目鱼肌、外周血流量(EPI)和 静脉注射马兜铃酸对腓肠肌的影响 ~3H-去甲基米帕明(DMI，血浆可溶示踪剂，摩尔质量分数=276)和 以DMI含量的比率(X(SD，n=8-9只/组))表示 肌肉/视网膜(一种不过度表达Glut-1的组织)。肌肉山梨醇 用气相色谱-质谱法测定其硼酸丁酯衍生物的含量。 这些观察表明，增加 通过山梨醇途径调节血糖升高的葡萄糖流量 骨骼肌流过表达GLUT-1。这些发现还包括 证明在山梨醇途径代谢方面存在显著差异 骨骼肌，以及骨骼肌中血流的变化 GLUT-1的过度表达不依赖于血浆胰岛素和血糖 级别。

The aim of this study was to assess the role of glucose metabolism via the sorbitol pathway in mediating increased blood flow in transgenic mice (6-8 wk. of age) overexpressing skeletal muscle GLUT-1. Blood flow was assessed soleus, epitrochlearis (epi), and gastrocnermius (gastroc) muscles by iv injection of 3H-desmethylimipramine (DMI, a plasma soluble tracer, mol wt=276) and expressed as a ratio (X(SD, n=8-9 mice per group) of DMI content in muscle/retina (a tissue not overexpressing GLUT-1). Muscle sorbitol levels were quantified by GCMS as their butylboronate derivatives. These observations suggest an important role for increased flux of glucose via the sorbitol pathway in mediating increased blood flow in skeletal muscles overexpressing GLUT-1. These findings also attest to marked differences in sorbitol pathway metabolism in skeletal muscles, and in blood flow changes in skeletal muscles overexpressing GLUT-1, independent of plasma insulin and glucose levels.