DESIGN & CONSTRUCT PELTIER DRIVEN COOLING & HEATING STAGE FOR LIGHT MICROSCOPE

（由NIH/NCRR/P41 RR 01219赠款支持）。 控制 在整个实验过程中，活体标本的温度，而 通过视频延时缩微观察， 他们的生存能力。 此外，在许多情况下， 要求快速冷却，然后重新加热细胞， 通过高分辨率视频光学显微镜观察。 例如在 某些哺乳动物细胞内的所有微管都可以 通过快速冷却至6 oC进行拆卸，然后重新加热， 微管从中心体重新长出来。 在某些情况下 希望能够解聚微管在一个特定的 细胞，然后在观察细胞的同时用激光破坏其中心体 显微外科手术或通过光学捕获来操纵它。 为此我们 设计并建造了一个廉价的珀耳帖驱动冷却系统 加热显微镜载物台。 载物台可以在内部冷却样品 玫瑰或灌注室（即使在油浸条件下观察 物镜）在不到6分钟的时间内升至~ 4xC，保持在此温度 温度下持续所需的时间（>60分钟），然后在需要时 将样本加热到稳态温度。 珀尔帖装置 允许由于可被加热的大（T）而快速冷却/加热。 在设备和样本之间产生。 这个特点， 当耦合到热传递函数的可逆性时， Peltier设备本身成本低（<20.00美元）使这是一个 实验台上理想的温度控制台 光学显微镜，要求快速，控制和持续 温度变化 里德尔角L.和R.W.科尔。 1998. 灌注 高分辨率视频光显微镜研究室 脊椎动物细胞单层：一些考虑和设计。 在 “视频显微镜”，G. Sluder和D. E.沃尔夫，埃德。Methods in Cell 生物学56：253-275。

(Supported by the NIH/NCRR/P41 RR 01219 grant). Controlling the temperature of living specimens throughout an experiment, and while viewing by video time-lapse microcopy, is fundamental to maintaining their viability. In addition, in many cases an experimental protocol calls for rapidly cooling and then re-warming a cell while it is being viewed by high resolution video-light microscopy. For example, in certain mammalian cells all microtubules within the cell can be disassembled by rapidly cooling it to 6oC, and re-warming then allows the microtubules to re-grow from the centrosome. In some cases it is desirable to be able to de-polymerizes the microtubules in a specific cell, and then while viewing the cell destroy its centrosome by laser microsurgery or manipulate it by optical trapping. To this end we have designed and constructed an inexpensive Peltier-driven cooling and heating microscope stage. The stage can cool the specimen within a Rose or perfusion chamber (even when viewed with oil immersion objectives) to ~ 4xC in less than 6 minutes, hold it at this temperature for as long as needed (>60 min), and then when desired warm the specimen to homeostatic temperature. The Peltier device allows for the rapid cooling/heating due to the large (T that can be generated between the device and the specimen. This characteristic, when coupled to the reversibility of heat transfer function and the low cost of the Peltier devices themselves (<$20.00 ea.) makes this an ideal temperature control stage for experiments on the stage of a light microscope that call for rapid, controlled and sustained temperature shifts. Rieder, C. L. and R.W. Cole. 1998. Perfusion chambers for high-resolution video-light microscopic studies of vertebrate cell monolayers: some considerations and a design. In "Video Microscopy", G. Sluder and D. E. Wolf, Eds. Methods in Cell Biology 56:253-275.