BIOCHEMICAL CHAR OF PROTEINS IN NGF SIGNAL TRANSDUCTION IN PC12 CELLS

神经生长因子（NGF）是一种靶源性因子， 与其受体TrkA在轴突尖端，发挥其作用， 神经元分化和存活。 到目前为止，人们对 轴突中的神经生长因子信号如何传递到细胞体。 最近 有证据表明，神经元利用小泡， 激活的NGF/TrkA复合物，以将NGF信号从 远轴突到细胞核，基因表达的变化是 诱导。 该项目的目的是检查的发生和身份， 这些蛋白质与活化的NGF/TrkA相关， 核内体 为此，将生成囊泡的蛋白质图谱 用2-D凝胶电泳， 质谱法将用于鉴定差异凝胶点。

Nerve growth factor (NGF) is a target-derived factor and interacts with its receptor, TrkA, on the axon tip, to exert its effects on neuronal differentiation and survival. To date, little is known on how NGF signalling in axons is communicated to the cell body. Recent evidence suggests that neurons exploit small vesicles that contain activated NGF/TrkA complexes, to convey the NGF signal from the distant axon to the nucleus, where changes in gene expression are induced. Aim of the project is to examine occurrence and identity of proteins that are associated with activated NGF/TrkA in these endosomes. To this end, protein maps will be generated of vesicles from NGF-treated versus control cells, using 2-D gel electrophoresis, and mass spectrometry will be used to identify differential gel spots.