STRUCTURE REFINEMENT OF MUTANT VERSIONS OF E COLI ASPARTATE TRANSCARBAMOYLASE

在这个实验室进行的项目集中在以下主题上 T细胞受体结构/功能相关性和信号传导 效应T细胞中的转导途径。 几个V* 基因已经被 识别，用于识别已定义的 同种特异性 肿瘤特异性T细胞系，其表达 特定T细胞受体已用于鉴定肿瘤特异性 抗原 这已经导致了粘蛋白分子的鉴定 （人粘蛋白muc-1）由导管上皮细胞表达，以及由 能够刺激T细胞的糖基化形式改变的肿瘤 免疫力 粘蛋白的结构通过NMR表征， 圆二色性以选择最佳疫苗，或 乳腺癌和胰腺癌免疫抑制抗原。 的 我们在两个系统中研究的效应T细胞对抗原 通过激活几种细胞内信号传导途径刺激。 我们特别感兴趣的是PKC途径作为一种手段， 抑制和控制T细胞活化。

The projects pursued in this laboratory center on the topics of structure/function correlation of T cell receptors, and signal transduction pathways in effector T cells. Several V* genes have been identified which are used to carry out recognition of defined allospecificities. Tumor specific T cell lines which express a particular T cell receptor have been use to identify tumor specific antigens. This has already led to identification of a mucin molecule (human mucin muc-1) expressed by ductal epithelial cells, and by tumors in an altered glycosylation form capable of stimulating T cell immunity. The structure of mucin is being characterized by NMR and circular dichroism in order to select the optimal vaccine or immunotherapeutic antigen for breast and pancreatic cancer. The effector T cells which we study in both systems respond to antigen stimulation by activating several intracellular signaling pathways. We are especially interested in the PKC pathway as a mean of pr omoting and controlling T cell activation.