ARSENITE STIMULATED OXYGEN RADICAL ON NITRIC OXIDE RELEASE FROM ENDOTHELIAL CELL

亚砷酸盐刺激氧自由基对内皮细胞释放一氧化氮的影响

• 批准号: 6206518
• 负责人: Aaron Barchowsky
• 金额: $ 0.61万
• 依托单位: DARTMOUTH COLLEGE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-01 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6206518
• 关键词: bioengineering /biomedical engineering; biological products; biomaterials; biomedical resource; gas; technology /technique

Carbon based paramagnetic materials are frequently used for EPR oximetry, especially in vivo, but the EPR spectra of these materials often show more than one paramagnetic center and/or relatively low signal intensity. To determine whether the components of carbon based materials could be separated and enriched in the active component, we used density gradient centrifugation to separate the materials into several fractions. We studied two types of coals, gloxy and Pocahontas, and found these materials to have large density distribution. The separated density fractions had very different EPR spectra and intensities. The active component from the coal material displays a more homogeneous EPR signal, and significantly increased EPR signal intensity. In similar studies with India ink, only slight changes were observed in attempts to separate the material. These results with coals may be very useful in the development of better probes for EPR oximetry.

碳基顺磁材料常用于EPR 血氧饱和度，特别是在体内，但这些材料的EPR谱 通常显示出多于一个的顺磁性中心和/或相对低的顺磁性中心 信号强度 为了确定碳基的成分是否 材料可以分离和富集活性成分， 使用密度梯度离心法将材料分离成 几个分数。 我们研究了两种类型的煤，gloxy和 波卡洪塔斯发现这些物质的密度很大 分布 不同密度组分的EPR差别很大 光谱和强度。 煤原料中的活性成分 显示出更均匀的EPR信号， EPR信号强度。 在类似的印度墨水研究中， 在分离材料的尝试中观察到变化。 这些 结果与煤可能是非常有用的发展更好的 用于EPR血氧测定的探头。