SIALYLATED SURFACE ANTIGENS OF CAMPYLOBACTER SPP

弯曲杆菌的唾液酸化表面抗原

• 批准号: 6170564
• 负责人: Patricia Guerry
• 金额: $ 17.26万
• 依托单位: HENRY M. JACKSON FDN FOR THE ADV MIL/MED
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6170564
• 关键词: Helicobacter; bacteria infection mechanism; carbohydrate biosynthesis; ferrets; flagellin; gene expression; gene mutation; genetic strain; genetic transcription; glycosylation; isoelectric point; laboratory rabbit; polymerase chain reaction; posttranslational modifications; protein structure; sialate; site directed mutagenesis; surface antigens; virulence

DESCRIPTION (Adapted from the Applicant's Abstract): Guerry and colleagues, in a first amendment to a new R01 application, propose to study the genetics of Campylobacter coli and C. jejuni genes responsible for post-translational glycosylation of flagellin. They propose that the modified surface-exposed protein plays a role in serospecificity of strains and serves as important antigens, potentially useful for vaccine development. The approach will include molecular genetic analysis of genes involved in sialic acid biosynthesis and addition of these residues to flagellin. In vitro invasion assays and animal (ferret and rabbit) models of infection will be used to assess virulence of C. coli and C. jejuni glycosylation-deficient mutants. There are three specific aims. 1. Complete the characterization of the post-translational modification genes of flagellin in C. coli VC167, and correlate the genetics with the structure of the post-translational modification; 2. Characterize and mutate sialic acid biosynthetic genes in two strains of C. jejuni, MSC57360 and 81-176; and 3. Measure the virulence and immunogenicity of mutants of VC167, MSC, and 81-176 which are defective in post-translational modification genes and/or have non-sialylated LPS.

描述（改编自申请人的摘要）：Guerry及其同事，在 对新R 01申请的第一次修正，建议研究 大肠弯曲杆菌（Campylobacter coli）和C.负责翻译后的空肠基因 鞭毛蛋白的糖基化。他们提出，改性的表面暴露 蛋白质在菌株的血清特异性中起作用， 抗原，可能用于疫苗开发。其办法将包括 参与唾液酸生物合成的基因的分子遗传学分析， 将这些残基添加到鞭毛蛋白中。体外侵袭测定和动物实验 （雪貂和兔）感染模型将用于评估C. coli和C.空肠糖基化缺陷突变体。有三个具体的 目标。1.完成翻译后修饰的表征 鞭毛蛋白基因在C. coliVC 167，并将遗传学与 翻译后修饰的结构; 2.特征化和变异 唾液酸生物合成基因在两株C.空肠，MSC 57360和 81-176; 3.测定VC 167突变体的毒力和免疫原性， MSC和81-176，它们在翻译后修饰基因中有缺陷 和/或具有非唾液酸化的LPS。