"Establishing fast superresolution live cell and tissue imaging with photomanipulation"

人体免疫系统对积极健康和解决疾病至关重要，但缺乏相关信息。近年来，免疫反应依赖于其器官、免疫细胞和分子的多尺度关系，这一观点在生物医学科学中已被广泛接受，但一个整体的观点仍然难以捉摸。多年来，显微镜一直是免疫反应科学发现的一个来源。然而，直到最近，还没有显微镜技术具有适当的灵敏度来研究跨尺度的免疫反应。该MRC中程设备应用程序的重点是这一挑战的核心，旨在建立蔡司Elyra 7 SIM 2与定制的光电处理，它提供了快速定量超分辨率的生物成像的活组织的尺度小到细胞和分子。蔡司Elyra提供的特殊光电操作功能允许光激活/光笼/光漂白，从而跟踪周围组织环境中的特定分子和/或细胞。我们在多个场合测试了ZEISS Elyra 7，发现该仪器非常适合提出的研究问题，设计良好，与定制的基于sim的显微镜获得的实验直接比较，用户友好。ZEISS Elyra 7在这方面尤其令人印象深刻，它允许人们轻松地安装和定位样品，并在非常稳定的条件下保持SIM，适合于扩展单细胞和薄10-100um组织切片生物成像。据我们所知，这将是唯一的蔡司Elyra 7 SIM 2平台与图片操纵在英国。蔡司Elyra 7 SIM2具有图像处理功能，满足组织界多年来的所有要求。蔡司Elyra 7的用户群来自MRC和wellcome资助的研究人员组成的联盟，将在UKRI-MRC的职责范围内进行研究，重点是感染和免疫、全球健康、神经科学和心理健康、MRC对COVID的反应以及转化医学。蔡司Elyra 7的MRC中程设备成功应用将大大扩展牛津和英国生物成像的广度、容量和能力，使英国的生物成像能力保持在国际研究的前沿。

Human immune system is critical for active health and for resolving disease, but information is missing. Over recent years it has become widely accepted in the biomedical sciences that the immune response depends on the multiscale relationships of its organs, immune cells, and molecules, but a holistic perspective remains elusive. Microscopy has, for many years, been a source of scientific discovery for the immune response. However, until recently there has been no microscopy technology with the right sensitivity to study the immune response across scales. The focus of this MRC Mid-range Equipment application sits at the heart of this challenge aiming to establish the ZEISS Elyra 7 SIM 2 with custom-built photomanipuation, which offers fast quantitative superresolution bioimaging of living tissues across scales down to cells and molecules. The special photomanipulation feature provided by this ZEISS Elyra with photomanipulation allows to photoactivate/photouncage/photobleach and thus track specific molecules and/or cells in the surrounding tissue context.We tested the ZEISS Elyra 7 at multiple occasions and found the instrument to be ideal for the proposed research questions, well engineered, and user friendly in direct comparison with experiments acquired at bespoke SIM-based microscopes. The ZEISS Elyra 7 was particularly impressive in this respect, allowing one to easily mount and locate samples, as well as maintain SIM under very stable conditions suitable for extended single cell and thin 10-100um tissue slice bioimaging. To the best of our knowledge, this will be the only ZEISS Elyra 7 SIM 2 platform with photomanipulation in the UK.The Zeiss Elyra 7 SIM2 with photomanipulation meets all the requirements demanded by the tissue community for years. The user base for the ZEISS Elyra 7 with photomanipulation stems from a consortium of MRC-funded and Wellcome-funded investigators and will be undertaking research within UKRI-MRC remit, focusing on infections and immunity, global health, neuroscience and mental health, MRC response to COVID, and translational medicine.A successful MRC Mid-range Equipment application for the ZEISS Elyra 7 would significantly expand the breadth, capacity, and capabilities of bioimaging in Oxford and in the UK, keeping UK's bioimaging abilities at the forefront of international research.