Field Emission Gun Scanning Electron Microscope

场发射枪扫描电子显微镜

• 批准号: MR/X012867/1
• 负责人: Lucy Margaret Collinson
• 金额: $ 101.1万
• 依托单位: The Francis Crick Institute
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FX012867%2F1
• 关键词: Field; Emission; Gun; Scanning; Electron

The Crick is a partnership between the Medical Research Council, Cancer Research UK, the Wellcome Trust and three leading universities: University College London, Imperial College London and King's College London. The Crick aspires to be one of the world's leading medical research institutes. The Crick achieves operational and research efficiencies and economies-of-scale through centralised facilities and functions, known as Science Operations, that provide all researchers at the Crick, irrespective of affiliation, with access to cutting-edge equipment, animals for research and laboratory enabling functions such as the Electron Microscopy Science Technology Platform (EM STP). Electron microscopy is a fundamental tool in bioscience research. It is used to image the structure of proteins, pathogens, cells and tissues, to help us understand the biological processes of normal development, disease states, and the effect of therapies.This proposal is for a new scanning electron microscope that will be used for state-of the-art imaging experiments for more than 60 research projects ongoing at the Crick. The microscope can image the surface of cells and tissues to reveal, for example, how immune cells fight infection, how nerve cells connect and communicate, and how drugs work against a range of infectious agents. The microscope has a range of additional features, including automated image stitching to allow us to view large samples and digitally zoom into features of interest (like 'google maps'); faster, more automated, three-dimensional imaging of samples using a technique called array tomography; and a cryo-stage for imaging samples encased in ice, as close to their living state as possible.

克里克是医学研究委员会、英国癌症研究中心、惠康信托基金会和三所顶尖大学（伦敦大学学院、伦敦帝国理工学院和伦敦国王学院）之间的合作伙伴关系。克里克医学院立志成为世界领先的医学研究机构之一。克里克通过被称为科学操作的集中设施和功能实现了运营和研究效率和规模经济，这为克里克的所有研究人员提供了最先进的设备，研究动物和实验室功能，如电子显微镜科学技术平台（EM STP）。电子显微镜是生物科学研究的基本工具。它被用来成像蛋白质、病原体、细胞和组织的结构，帮助我们了解正常发育、疾病状态和治疗效果的生物过程。这是一个新的扫描电子显微镜的建议，它将用于克里克正在进行的60多个研究项目的最先进的成像实验。这种显微镜可以对细胞和组织表面进行成像，以揭示免疫细胞如何对抗感染，神经细胞如何连接和交流，以及药物如何对抗一系列感染因子。显微镜有一系列额外的功能，包括自动图像拼接，使我们能够查看大样本和数字放大到感兴趣的特征（如“谷歌地图”）；使用阵列断层扫描技术对样品进行更快、更自动化的三维成像；还有一个冷冻阶段，用于将样品包裹在冰中成像，尽可能接近它们的生活状态。