6-laser high specification spectral flow cytometer analyzer (Sony ID7000)

6激光高规格光谱流式细胞仪分析仪（Sony ID7000）

• 批准号: MR/Y001990/1
• 负责人: Pier Andree Penttila
• 金额: $ 68.46万
• 依托单位: MRC Laboratory of Molecular Biology
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FY001990%2F1
• 关键词: laser; specification; spectral; flow; cytometer

The body is composed of billions of cells which have many different functions depending on the organs which they make up or the body tissues in which they reside. Cells may also change during their lifetime e.g. immune cells responding to infections or mutations in cells may lead to cancer. To be able to identify differences and changes in cells we need sensitive machines like fluorescence activated cell analysers. These scientific instruments shine light onto cells which have been labelled with antibodies that glow if they are lit up. If the cell glows then it indicates the presence of a specific protein on the cell. Many colours can be used to detect a multitude of proteins thereby giving a "single cell signature". Our research will investigate how these signatures can be used to predict diseases e.g. abnormal embryos, changes in the immune system, inflammation, cancer.

人体由数十亿个细胞组成，这些细胞有许多不同的功能，这取决于它们组成的器官或它们所处的身体组织。细胞也可能在其一生中发生变化，例如免疫细胞对感染作出反应，细胞中的突变可能导致癌症。为了能够识别细胞的差异和变化，我们需要像荧光激活细胞分析仪这样灵敏的机器。这些科学仪器将光线照射到标记有抗体的细胞上，这些抗体在被照亮时会发光。如果细胞发光，则表明细胞上存在特定的蛋白质。许多颜色可以用来检测多种蛋白质，从而给出“单细胞特征”。我们的研究将探讨如何利用这些特征来预测疾病，如胚胎异常、免疫系统变化、炎症、癌症。