FUNDAMENTALS OF BIOLOGICAL ELECTRON TRANSFER

描述：氧化还原酶功能障碍被认为是一种 对几种常见疾病的衰弱有重大贡献， 非常复杂，治愈的可能性很小 了解 不同种类的氧化还原酶的功能原理，以及 他们的工程公差和故障阈值几乎 当然有助于改善疾病的影响， 提供急需的诊断工具，并指出方向， 疗方 这一建议旨在了解一个基本部分， 许多氧化还原酶，即电子的传递， 催化或能量耦合位点。 PI计划应用共识 从我们对天然氧化还原蛋白质的研究中提取的一般性 结构和电子隧穿功能的设计，从头开始 简化的氧化还原蛋白的合成和组装;即，模型 通过检测相对简单稳定的天然氧化还原中心， 结构表征的，异质肽环境， maquettes，他希望描述的基本事件，是耦合在 平衡到常见氧化还原辅因子的氧化和还原。 氧化还原辅因子将被检查，无论是单独的，当整合 在多个氧化还原辅因子链模型中。 私家侦探计划启动 蛋白质内电子和自由基通过这些链转移， 光、氧或在电极/肽处用电学方法 单层界面 这些模型的目的是最终访问 控制电子转移的动力学因素，并揭示了 平衡性质在动力学时间尺度上表现出来。

DESCRIPTION: Oxidoreductase dysfunction is becoming recognized as a major contribution to the debilitation of several common diseases that are highly complex with poor prospects for cure. Understanding the principles of function of the different classes of oxidoreductases, and their engineering tolerances and the thresholds of failure would almost certainly help in ameliorating the effects of the illnesses, by providing much needed diagnostic tools and pointing the direction to a cure. This proposal is aimed at understanding a fundamental part of many oxidoreductases, namely the delivery of electrons to and from catalytic or energy coupling sites. The PI plans to apply the consensus and generality extracted from our examination of natural redox protein construction and electron tunneling function to the design, de novo synthesis and assembly of simplified redox proteins; i.e., maquettes. By examing natural redox centers in the relatively simple stable, structurally characterized, heterogeneous peptide environment of maquettes, he hopes to describe the basic events that are coupled at equilibrium to the oxidation and reduction of common redox cofactors. Redox cofactors will be examined, both individually, and when integrated in multiple redox cofactor chain maquettes. The PI plans to activate intraprotein electron and radical transfer through these chains with light, oxygen or with electrical methods at the electrode/peptide monolayer interface. These maquettes are designed to ultimately access the kinetic factors that govern electron transfer and reveal how the equilibrium properties manifest themselves on the kinetic timescale.