DEVELOPMENT OF A NORMAL HUMAN HEPATOCYTE CELL LINE

正常人肝细胞系的发育

• 批准号: 6074632
• 负责人: JING WANG
• 金额: $ 15.88万
• 依托单位: GENETICA, INC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-30 至 2000-09-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6074632
• 关键词: Retroviridae; cell line; gene targeting; liver cells; technology /technique development; tissue /cell culture; transfection /expression vector

DESCRIPTION: (Adapted from the Investigator's Abstract) It was initially proposed that telomerase alone could immortalize human cells without deleterious effects on the genome. In the recent past, these investigators and other researchers have demonstrated that telomerase can only immortalize epithelial cells that have already escaped from MO, a telomere-independent mortality control parameter. Furthermore, escape from MO is accompanied by inactivation of the p16/Rb tumor suppressor pathway. Knowledge of the effectors of MO (e.g. p16 gene), and of regulators of telomerase (e.g. myc) suggested that binary genetic combinations could significantly effect single-step immortalization. Currently, we lack a novel technique of immortalizing human cells for therapeutic use. The investigators have already conducted preliminary experiments and established single step immortalization of human keratinocytes. PROPOSED COMMERCIAL APPLICATION: NOT AVAILABLE

描述：(改编自《调查者摘要》)最初 提出仅靠端粒酶就可以使人类细胞永生 对基因组的有害影响。在最近的过去，这些调查人员和 其他研究人员已经证明，端粒酶只能永生 已经从端粒非依赖性的MO中逃脱的上皮细胞 死亡率控制参数。此外，逃离MO伴随着 P16/Rb肿瘤抑制通路失活。效应器的知识 MO(如p16基因)和端粒酶调节因子(如myc) 二元遗传组合可以显著影响单步 永垂不朽。目前，我们缺乏一种使人类永生的新技术 用于治疗的细胞。调查人员已经进行了初步的 实验并建立了人角质形成细胞的一步永生化。 建议的商业应用：不可用